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Evaluation of nanoparticle-based plasma enrichment on individuals with primary and metastatic pancreatic cancer

ABSTRACT: Using nanoparticles based plasma enrichment strategies on plasma samples, we were able to achieve a huge increase of identified plasma proteins from around 700 to more than 5000 proteins as compared to neat plasma digest. The substantial increase in depth allowed us to perform in-depth analysis and we were able to apply this to a small cohort of plasma samples from pancreatic cancer (PC) patients with primary tumor, tumor that had metastases vs healthy controls. Majority of identified proteins were part of the Human Plasma Proteome Project (HPPP) database, and more than 300 proteins are on the list of FDA approved drug targets. We showed a large and significant increase in ribosomal proteins in plasma of PC that metastasized. ADH1C and ADH1B, both members of the alcohol dehydrogenase family, had one of the largest increase and potentially correlated with liver metastasis. We further highlighted 15 different potentially secreted and/or cell surface proteins that were otherwise not identified in neat, digested plasma and have potential to be useful markers due to their cancer associations. Lastly, we compared different mass spectrometer (Orbitrap Astral and Orbitrap Ascend) and column (depth and throughput) setups on the same dataset and showed similar conclusions potentially can be achieved, although the depth approach on the newer instrumentations can reveal additional insights in the plasma proteome.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

DISEASE(S): Pancreatic Cancer

SUBMITTER: Ching-Seng Ang

LAB HEAD: Ching-Seng Ang

PROVIDER: PXD065103 | Pride | 2025-12-03

REPOSITORIES: Pride

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			Action	DRS
	250409_ACS_Ascend_Nano_Plas_1.raw	Raw
	250409_ACS_Ascend_Nano_Plas_10.raw	Raw
	250409_ACS_Ascend_Nano_Plas_11.raw	Raw
	250409_ACS_Ascend_Nano_Plas_12.raw	Raw
	250409_ACS_Ascend_Nano_Plas_13.raw	Raw

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Evaluation of nanoparticle-based plasma enrichment on individuals with primary and metastatic pancreatic cancer

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