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Proteome-wide identification of ISG15 sites in HeLa cells

ABSTRACT: We employed a GlyGly-peptidomics approach to comprehensively map ISG15 modification sites in HeLa cells under three conditions: mock-transfected controls, IFN-induced cells, and cells transfected with the ISGylation machinery. Lysates from wild-type and Isg15 knockout (KO) cells were processed using S-trap, followed by trypsin digestion to generate diglycine-modified peptides. These peptides were enriched via immunoprecipitation and analyzed by LC-MS/MS for identification and quantification. Comparative analysis across conditions and genotypes revealed 671 ISG15 modification sites on 456 distinct proteins, expanding the HeLa ISGylome.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell

SUBMITTER: Denzel Eggermont

LAB HEAD: Francis Impens

PROVIDER: PXD065158 | Pride | 2026-03-02

REPOSITORIES: Pride

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			Action	DRS
	B2652_Ap_IonOpt_CMB-1265_FRIMP_GlyGly_Lissa-1.raw	Raw
	B2654_Ap_IonOpt_CMB-1265_FRIMP_GlyGly_Lissa-2.raw	Raw
	B2656_Ap_IonOpt_CMB-1265_FRIMP_GlyGly_Lissa-3.raw	Raw
	B2658_Ap_IonOpt_CMB-1265_FRIMP_GlyGly_Lissa-4.raw	Raw
	B2660_Ap_IonOpt_CMB-1265_FRIMP_GlyGly_Lissa-5.raw	Raw

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