The immune response against cancer is modulated by stromal cell fibronectin

ABSTRACT: The data set compares murine bone marrow stromal cells that express osterix-cre (and hence cells in which the osterix promoter is active) to the cells isolated from the same animals that did not express osterix-cre (and hence do not activate the osterix promoter). To sort these cells, we used the reporter gene tdTomato. If the gene is present, the protein is not expressed because the gene is preceded by a STOP codon flanked by two floxed sites. If, on the other hand, cre is expressed (in this case osterix-cre) in the same cell that contains the tdTomato gene, activation of the promoter will lead to cre recombinase expression and removal of the gene part flanked by floxed sequences. This will remove the STOP codon and lead to expression of the tdTomato protein. Cells that are osterix-cre_tdTomatofloxed/+ therefore will express tdTomato and can be detected by flow cytometry allowing sorting. The cells were isolated from the bone marrow of adult mice. It should be noted, that in addition to the difference in osterix promoter activation, tdTomato is also expressed in the sorted osterix-cre cells. There are 4 samples that do not express osterix followed by 4 samples that do as follows: Control samples numbered 1-4 and Osterix samples numbered 5-8. Analysis results are summarized as supplementary figure 3 and supplementary table 1 in Lubosch, Pitt et al. 2025, Neoplasia

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Barbara Steigenberger  

LAB HEAD: Inaam Nakchbandi

PROVIDER: PXD065221 | Pride | 2025-06-20

REPOSITORIES: Pride