Dataset Information

MS analyses of mouse VLDL from WT and ApoA5 (Apolipoprotein A-V) knockout mice

ABSTRACT: The impact of APOA5 on VLDL composition and its potential to alter VLDL metabolism in other ways remains poorly understood. To address this, we investigated the influence of APOA5 on the VLDL proteome. We used VLDL from Apoa5 knockout (KO) and wild-type (WT) mice, for mass spectrometry utilizing two independent laboratories. We identified consistent depletion of 17 proteins involved in lipoprotein metabolism, inflammation, and immune response in Apoa5 KO VLDL, including APOE and serum amyloid A1 (SAA1).

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Blood Plasma

SUBMITTER: Pheruza Tarapore

LAB HEAD: W. Sean Davidson

PROVIDER: PXD065274 | Pride | 2025-11-24

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	1064324_VLDL_ApoA5_wt_4_02Aug22_Oak_WBEH_22-06-16.raw	Raw
	1064351_VLDL_ApoA5_KO_4_02Aug22_Oak_WBEH_22-06-16.raw	Raw
	1064399_VLDL_ApoA5_wt_1_02Aug22_Oak_WBEH_22-06-16.raw	Raw
	1064423_VLDL_ApoA5_wt_2_02Aug22_Oak_WBEH_22-06-16.raw	Raw
	1064446_VLDL_ApoA5_KO_3_02Aug22_Oak_WBEH_22-06-16.raw	Raw

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Publications

Apolipoprotein A5 reduces clearance of VLDL by altering apolipoprotein E content.

Tarapore Pheruza P Swertfeger Debi D Morris Jamie J He Yi Y Sarkar Snigdha S Melchior John T JT Shah Amy S AS Liu Min M Davidson W Sean WS

Journal of lipid research 20251001 11

Apolipoprotein A-V (APOA5) is a critical regulator of circulating triglyceride (TG) levels. Its deletion leads to elevated plasma TG concentrations by altering the metabolism of VLDL particles in vivo. One way APOA5 exerts its effects is through the modulation of LPL activity, specifically by disrupting inhibitory interactions between LPL and angiopoietin-like proteins (ANGPTLs). However, the impact of APOA5 on VLDL composition and its potential to alter VLDL metabolism in other ways remains poo ...[more]

PMID: 41043689

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