Proteome integral solubility alteration assay for monitoring antibody-antigen interactions in cells

ABSTRACT: Proteome Integral Solubility Alteration (PISA) assay is normally used for characterization of the interactions between a small molecule and the cellular proteome. Here we modified PISA uniquely to identify the antigen (tumor necrosis factor alpha) in activated monocytes using the monoclonal antibody Infliximab.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Monocyte

SUBMITTER: Susanna Lundstrom  

LAB HEAD: Roman Zubarev

PROVIDER: PXD065457 | Pride | 2026-05-20

REPOSITORIES: Pride