Dataset Information

TgGPN1 interactome from Toxoplasma gondii tachyzoites

ABSTRACT: TgGPN1 interactome from Toxoplasma gondii tachyzoites was analyzed in 3 Biological Replicates, with 3 Technical Replicates each, by comparing GPN1-HA tagged strain against parental, non-tagged strain in 2 genetic backgrounds (WT and SPY_KO).

INSTRUMENT(S):

ORGANISM(S): Toxoplasma Gondii Gt1

TISSUE(S): Cell Culture, Fibroblast

DISEASE(S): Toxoplasmosis

SUBMITTER: Christopher West

LAB HEAD: Christopher M. West

PROVIDER: PXD065533 | Pride | 2025-09-19

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	GPN_HA_RH_SPY_3_BioRep.mzML	Mzml
	GPN_HA_RH_SPY_3_BioRep.mzid	Mzid
	GPN_HA_in_RH_10ul_09Jan25_1.raw	Raw
	GPN_HA_in_RH_10ul_09Jan25_2.raw	Raw
	GPN_HA_in_RH_10ul_09Jan25_3.raw	Raw

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Publications

O-fucosylation affects abundance but not localization of select nucleocytoplasmic proteins in toxoplasma gondii.

Tiwari Megna M Gas-Pascual Elisabet E Teal-Urquides Janice J Samuelson John J West Christopher M CM

Glycobiology 20250901 10

Toxoplasma gondii is a highly successful intracellular mammalian and avian pathogen that must adapt to a wide range of intracellular and extracellular environments. A mechanism that may support this is the modification of hydroxyamino acid rich sequences of nucleocytoplasmic proteins with O-fucose. O-fucosylation of possibly hundreds of proteins is mediated by a single highly conserved nucleocytoplasmic enzyme. Deletion of the SPY O-fucosyltransferase gene is tolerated but inhibits parasite prol ...[more]

PMID: 40888172

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Project description:Toxoplasma gondii is a globally distributed obligate intracellular parasite which can cause zoonotic toxoplasmosis with great harms. The average death time of mice that infected with Toxoplasma gondii RH strain tachyzoites recovered from the liquid nitrogen was shortened after multiple generations. It has been reported that the parasite is in a state of static virulence during cryopreservation and the virulence of the protozoan parasite can be enhanced after continuous passages in hosts under laboratory conditions. However, no research has been conducted to elucidate its biological mechanism. Herein, we sequenced the T. gondii transcriptome using RNA-Seq technology and performed de novo assembly to investigated the virulence factors expression changes by comparing gene expression profiles between incipiently recovered and completely resuscitated tachyzoites. Transcriptome analysis identified 1,951 differentially expressed transcripts in infected liver, of which 1,752 were significantly downregulated and 199 upregulated. We identified many differentially expressed proteins and genes, including serine/threonine kinase, calnexin, myosin and microtubule-associated protein which have previously been reported to be either involved in cell adhesion, parasite gliding or participate in cell invasion. The great majority of the virulence factors including microneme proteins, rhoptry proteins and dense granule proteins were upregulated in fully recovered tachyzoites. The enhanced virulence of recovered Toxoplasma gondii RH strain from the liquid nitrogen is associated with the up-regulated expression of MICs, ROPs and GRAs. Our data will facilitate future genomic research and in-depth annotation of Toxoplasma gondii RH strain genomes. This study provides a profile of the candidate genes that are suspected to be involved with virulence enhancement of recovered Toxoplasma gondii RH strain tachyzoites. Many further studies should be carried out to confirm the function of the candidate genes. Moreover, the preliminary identification of genes and pathways exhibiting differential expression in complete resuscitation stage may further our general understanding of virulence enhancement in this parasite.