Ubiquitination modification omics

ABSTRACT: Protein ubiquitination plays a vital role in the stress response of diverse filamentous fungi. However, few reports are available on fungal insect pathogens including Metarhizium. Here, we report a comparative ubiquitylome analysis of M. robertsii exposed to heat stress. The growth of M. robertsii was suppressed and protein ubiquitination levels were markedly promoted during heat stress. Compared to the control treatment, there were 4,674 sites with differential ubiquitination, of which 3,419 lysine ubiquitination sites across 1,344 proteins were significantly up-regulated, and 1,255 sites on 750 proteins were down-regulated under heat stress. Further analysis showed that these proteins with up-regulated modified sites were preferentially enriched in the phenylalanine, tyrosine, and tryptophan biosynthesis, pantothenate and CoA biosynthesis, and O-glycan biosynthesis pathways. Proteins with down-regulated modified sites were significantly enriched in different pathways including alanine, aspartate, and glutamate metabolism, pyruvate metabolism, and fatty acid biosynthesis. In particular, a key protein (phosphoenolpyruvate carboxykinase, MrPCK1, a central enzyme in gluconeogenesis and pyruvate metabolism) with five ubiquitination sites was identified, and functional analysis further revealed its regulatory role in heat stress tolerance of M. robertsii. Taken together, our findings suggest that M. robertsii may respond to heat stress not only through the canonical pathway of the proteasome but also by modulating specific metabolic pathways, including pyruvate metabolism (notably via MrPCK1) and potentially fatty acid biosynthesis. The results provide insights into the molecular mechanisms by which ubiquitination regulates the heat stress response in M. robertsii and contribute to our understanding of thermotolerance in filamentous fungi.

INSTRUMENT(S):

ORGANISM(S): Metarhizium Robertsii Arsef 2575

TISSUE(S): Conidium, Mycelium

SUBMITTER: Jueping Song  

LAB HEAD: Jueping Song

PROVIDER: PXD065615 | Pride | 2025-11-26

REPOSITORIES: Pride