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Influence of bead chemistry and protein-to-bead ratio on the efficiency of solid-phase proteolysis

ABSTRACT: Optimization of an SP3 digestion workflow on human plasma samples. The performance of the SP3 workflow using beads with different surface chemistries (SpeedBeads and MagReSyn beads) was evaluated.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

SUBMITTER: Jean ARMENGAUD

LAB HEAD: Jean Armengaud

PROVIDER: PXD065651 | Pride | 2026-02-24

REPOSITORIES: Pride

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			Action	DRS
	E24392_Speedbeads_1_4_0_5_1.raw	Raw
	E24393_Speedbeads_1_4_0_5_2.raw	Raw
	E24394_Speedbeads_1_4_0_5_3.raw	Raw
	E24396_Speedbeads_1_10_0_5_1.raw	Raw
	E24397_Speedbeads_1_10_0_5_2.raw	Raw

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Publications

Influence of Bead Chemistry and Protein-to-Bead Ratio on the Efficiency of Solid-Phase Proteolysis.

Al Siblani Salem S Armengaud Jean J Lozano Clément C

Journal of proteome research 20260106 2

Single-pot, solid-phase-enhanced sample preparation (SP3) is a rapid, automatable, and cost-effective protein cleanup technique facilitated by the aggregation and digestion of proteins on paramagnetic beads. In this study, we document how plasma protein-to-bead ratio influences the performance in terms of the number of identified peptides and proteins for two chemically modified paramagnetic SP3 beads, i.e., Carboxylate-Speedbeads and MagReSyn-Hydroxyl beads. The optimized protein-to-bead ratio ...[more]

PMID: 41493836

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