Dataset Information

Arabidopsis thaliana PSS1 TurboID N-teminal fusion

ABSTRACT: The Arabidopsis PSS1 gene, which encodes a glycine-rich protein, plays a pivotal role in nonhost resistance to the soybean oomycete Phytophthora sojae and the fungal pathogen Fusarium virguliforme. To elucidate the molecular basis of PSS1-mediated immunity, we employed miniTurbo-based proximity labeling in stable transgenic Arabidopsis lines expressing miniTurbo fused to either wild-type PSS1 or its nonfunctional mutant variant, PSS1G119D. Seedlings were challenged with or without P. sojae, and biotinylated proteins were isolated and analyzed by mass spectrometry. The resulting PSS1 interactomes revealed several candidate proteins predominantly localized in the plastid and cytosol. Notably, proteins such as LIN2, ATPMEPCRA, and TGG1 were specifically enriched in wild-type PSS1 lines under P. sojae infection, suggesting their potential involvement in PSS1-mediated nonhost resistance. Our findings offer novel insights into immune protein networks and provide a foundational resource for dissecting nonhost immunity mechanisms in plants

INSTRUMENT(S):

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress) Phytophthora Sojae

TISSUE(S): Seedling

SUBMITTER: Christian Montes Serey

LAB HEAD: Madann K. Bhattacharyya

PROVIDER: PXD065660 | Pride | 2026-06-11

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	0115_CMS_240229-OA-TMTp_FAIMS-TbID_N-4x1000ng_OE480-01.raw	Raw
	0115_CMS_240229-OA-TMTp_FAIMS-TbID_N-4x1000ng_OE480-02.raw	Raw
	0115_CMS_240229-OA-TMTp_FAIMS-TbID_N-4x1000ng_OE480-03.raw	Raw
	0115_CMS_240229-OA-TMTp_FAIMS-TbID_N-4x1000ng_OE480-04.raw	Raw
	Ath_TAIR10_pep_20101214.fasta	Fasta

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Publications

Proximity labelling suggests association of the nonhost receptor PSS1 protein with enzymes of multiple defense pathways in Arabidopsis.

Akintayo Oluwatoyosi F OF Montes Serey Christian C Walley Justin W JW Bhattacharyya Madan K MK

Frontiers in plant science 20260305

<h4>Introduction</h4><i>PSS1</i> encoding a glycine-rich integral plasma membrane protein plays a pivotal role in nonhost resistance of Arabidopsis against the soybean pathogens, <i>Phytophthora sojae</i> and <i>Fusarium virguliforme</i>. Notably, <i>P. sojae</i> fails to penetrate the wildtype Col-0 ecotype but not the pss1 mutant. To elucidate the molecular basis of <i>PSS1</i>-mediated nonhost immunity, we employed a miniTurbo-based proximity labeling approach.<h4>Methods</h4>The miniTurbo fu ...[more]

PMID: 41869043

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