Proteomic analyse of equine tooth (dentin) ageing
Ontology highlight
ABSTRACT:
INSTRUMENT(S):
ORGANISM(S): Equus Caballus (horse)
TISSUE(S): Dentin
SUBMITTER: 
Alzbeta Chabronova
LAB HEAD: Mandy Peffers
PROVIDER: PXD065894 | Pride | 2026-05-13
REPOSITORIES: Pride
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| 220224_MP_eqdentine_1.mgf | Mgf | |||
| 220224_MP_eqdentine_1.mzid | Mzid | |||
| 220224_MP_eqdentine_1.raw | Raw | |||
| 220224_MP_eqdentine_10.mgf | Mgf | |||
| 220224_MP_eqdentine_10.mzid | Mzid |
Items per page: 5 1 - 5 of 45 |
Similar Datasets
Project description:This study investigates the proteomic profiles of horse tooth tissues, including pulp, dentin, cementum, and enamel, with a focus on the effects of age and disease. We analyzed tissue samples from young, old, and diseased teeth to explore how aging and disease influence protein expression across these dental tissues. The goal was to identify tissue-specific proteomic signatures and potential biomarkers associated with age-related changes and dental diseases. The findings offer insights into the molecular mechanisms underlying tooth aging and disease, providing a foundation for future studies on equine dental health and potential therapeutic targets.
2026-05-13 | PXD065888 | Pride
Project description:This study investigates the proteomic profiles of horse tooth tissues, including pulp, dentin, cementum, and enamel, with a focus on the effects of age and disease. We analyzed tissue samples from young, old, and diseased teeth to explore how aging and disease influence protein expression across these dental tissues. The goal was to identify tissue-specific proteomic signatures and potential biomarkers associated with age-related changes and dental diseases. The findings offer insights into the molecular mechanisms underlying tooth aging and disease, providing a foundation for future studies on equine dental health and potential therapeutic targets.
2026-05-13 | PXD066267 | Pride
Project description:This study investigates the proteomic profiles of horse tooth tissue, pulp, with a focus on the effects of age and disease. We analysed tissue samples from young, old, and diseased teeth to explore how ageing and disease influence protein expression across these dental tissues. The goal was to identify tissue-specific proteomic signatures and potential biomarkers associated with age-related changes and dental diseases. The findings offer insights into the molecular mechanisms underlying tooth ageing and disease, providing a foundation for future studies on equine dental health and potential therapeutic targets.
2026-05-13 | PXD067261 | Pride
Project description:Equine odontoclastic tooth resorption and hypercementosis (EOTRH) is a relatively recently described dental disorder, commonly found in horses above the age of 15 years old (Carsten Staszyk et al. 2008). The disease was first categorised as EOTRH in 2008, following the identification of clinical cases involving severe resorptive lesions and cementomas forming on the lower portion of equine incisors (Kreutzer et al. 2007) (Baratt 2007). The two main disease indicators are resorptive lesions caused by odontoclasts and hypercementosis, characterised by reparative/irregular cementum deposition, forming bulbous-like features (Schätzle, Tanner, and Bosshardt 2005). Horses with severe EOTRH often present dysphagia, pain whilst eating and behavioral changes, which may lead to further unrelated complications (Earley and Rawlinson 2013). Some suggested that Warmbloods and Thoroughbreds are more commonly diagnosed (Sykora et al, 2014; Smedley et al, 2015; Lorello et al, 2016; Earley et al, 2017), although others state there are no appar¬ent breed associations (James, 2022; Tretow et al, 2023). Furthermore, in a limited group of Icelandic horses, a diagnosis was established in 72.2% of cases (Tretow, Hain, and Bienert-Zeit 2023). While radiography is the primary diagnostic method for EOTRH after clinical examination, recent studies have indicated that this approach may lack sensitivity for early disease detection, potentially impeding treatment if the condition has advanced significantly (Albers, Bienert-Zeit, and Staszyk 2022). A comparable ailment observed in cats is Feline Odontoclastic Resorptive Lesions (FORL). In this species there was a prevalence of 29% , and similar to EOTRH, it exhibited an elevated risk associated with age (Gorrel 2014). The underlying causes of EOTRH are not yet fully understood with many potential factors considered, including age, sex, breed, diet and environment (Pearson et al. 2013). One of the first theories suggest a sequence of events beginning with periodontal inflammation leading to resorptive lesions and subsequent cementum production (Carsten Staszyk et al. 2008). Other studies found gram-negative bacteria (Treponema, Tannerella) were more commonly identified in the gingival crevicular fluid of diseased horses, highlighting the potential importance of the oral microbiome in disease aetiology (Sykora et al. 2013). Current treatment involves extraction of affected teeth. However there is an increased likelihood of neighboring teeth developing EOTRH (Rahmani et al. 2019). Presently there are no reported disease modifying treatments available to treat EOTRH and symptom management and pain reduction are the current gold standard approaches (Foster 2013). Whilst the cementum of teeth is the most effected dental tissue other types of dental tissues include dentine, enamel, pulp and the periodontium. The latter consists of cementum, periodontal ligament and alveolar bone. Compositionally cementum is similar to bone with 55% organic material and 45% inorganic hydroxyapatite (Tziafas 2005). It covers the entire length of equine teeth, potentially explaining the hyperactivity of cementum-producing cells (cementoblasts) in EOTRH [C. Staszyk, Suske, and Pöschke (2015)](Bosshardt 2005). Coronal cementum acts as a support to enamel, whilst cementum nearer to the roots is primarily an interface between tooth and the periodontal ligament (PDL). Finally a third type of cementum is present within the infundibulum near the coronal surface of the tooth. Irregular cementum has been shown to be deposited during EOTRH at both resorbed and non-resorbed surfaces along the tooth-PDL interface. Proteomic tools have been utilised to analyse clinical samples in both normal and disease-specific states. Specifically in dental tissues proteomics has been used to map proteomes of enamel, dentin, cementum and pulp in human teeth [Green et al. (2019)](Widbiller et al. 2019)[Giovani et al. (2020)](Eckhardt et al. 2014). As well as this human dental diseases, such as gingivitis, caries and periodontal disease have all been investigated with a similar approach [Gonçalves et al. (2011)](Wang et al. 2018)(Hartenbach et al. 2020). There have been no studies performed to identify potential pathological distinctions between the cementum tissues from healthy horses and those with EOTRH. We hypothesised that global protein changes in horses with and without EOTRH would increase the understanding of underlying pathways and disease mechanisms enabling insight into aetiologies. In addition, by understanding the pathophysiological mechanisms related to EOTRH this could provide treatment targets to delay or prevent disease progression. To investigate protein profiles we used unbiased label-free mass spectrometry-based proteomics.
2025-06-16 | PXD051310 | Pride
Project description:In this study, using mouse molar as the model, we developed a dual fluorescence reporter mouse to precisely track and analyze dental epithelium and mesenchyme at single-cell resolution from early embryonic to postnatal stages. Moreover, we constructed the virtual molar explorer (VMEx) to spatially map 15,967 molar-expressed genes and identified that Msx1+ Sdc1+ marked the developing dental papilla while surrounded by Msx1+ Sdc1- molar niche. Through tooth germ reconstitution and organoid culture in vitro and kidney capsule transplantation in vivo, we provided evidence that the Msx1+ Sdc1- dental follicle cells might function as the tooth organizers that promoted epithelium survival and tooth germ organization. Furthermore, the appearance of Msx1+ Sdc1+ dental papilla cells relied on the interaction between dental epithelium and Msx1+ Sdc1- dental follicle cells. Together, our results revealed the cellular dynamics of tooth development in mice and identified that the dental follicle might be the key driver of epithelial-mesenchymal interaction and tooth morphogenesis.
2022-11-01 | GSE162413 | GEO
Project description:Throughout the various stages of tooth development, reciprocal epithelial-mesenchymal interactions are the driving force, for instance crucially involved in the differentiation of mature enamel-forming ameloblasts and dentin-producing odontoblasts. Here we established mouse tooth ‘assembloids’, comprised of tooth organoid-derived dental epithelial cells (from mouse molars and incisors) cultured together with molar dental pulp stem cells (DPSCs), to mimic these developmental interactions. Assembloids from both tooth types were grown both in basal- and differentiation-inducing conditions. Single cell transcriptomics analysis was applied to in detail characterize and validate the newly developed mouse tooth assembloid model and evaluate the induced differentiation processes.
2023-04-19 | E-MTAB-12544 | biostudies-arrayexpress
Project description:Aging often triggers dental pulp fibrosis, leading to various clinical repercussions, including heightened vulnerability to dental infections, compromised tooth vitality, and reduced responsiveness to dental interventions. Despite its common occurrence in clinical settings, the precise molecular mechanisms driving this condition remain elusive. Leveraging single-cell transcriptome analysis from both our own and publicly available datasets, we identified Ccrl2+ macrophages as particularly prone to susceptibility during the early stages of aging. Notably, dental pulp progenitors exhibiting high expression of Rarres2, a unique ligand for Ccrl2, orchestrate the selective recruitment of a specific macrophage population to the stem cell niches. Subsequently, this results in the presentation of the ligand-receptor complex to Cmklr1, a receptor ubiquitously expressed among all macrophage populations, ultimately leading to macrophage activation and expansion via the Rarres2/Ccrl2/Cmklr1 axis. Our study, supported by rigorous experimental validation, conclusively shows that macrophage activation and expansion in stem cell niches lead to increased secretion of proinflammatory factors, which contribute to dental pulp fibrosis during aging. Our data reveals the complex molecular dynamics of dental pulp aging, particularly focusing on interactions within the immune microenvironment. It offers a novel perspective on potential treatments for age-related pulp diseases, highlighting the role of macrophages and the possibility of modifying the immune microenvironment for therapeutic benefit.
2025-01-29 | GSE253259 | GEO
Project description:During embryonic organogenesis, the odontogenic potential resides in dental mesenchyme from the bud stage until birth. Mouse dental mesenchymal cells (mDMCs) isolated from the inductive dental mesenchyme of developing molars are frequently used in the context of tooth development and regeneration. cultured mDMCs could retain the odontogenic potential for 24 h with a ratio of 60% for tooth formation, but mDMCs were incapable of supporting tooth formation after more than 24 h in culture. To reveal the underlying mechanism of the impairment of odontogenic potential, we generated gene expression profiles from mDMCs in culture using RNA-seq.
2016-10-31 | GSE78228 | GEO
Project description:The canonical bone morphogenetic protein (BMP) signaling pathway plays a crucial regulatory role in tooth development by activating Smad proteins to regulate gene expression. We previously identified an atypical canonical BMP signaling in dental mesenchyme that is Smad4-independent but Smad1/5-dependent. This study demonstrates that phosphorylated Smad1/5 (pSmad1/5) and Smad4 regulate distinct gene sets in dental mesenchyme. Real-time monitoring of BMP-Smad transcriptional activity revealed that Smad4-dependent canonical BMP signaling is restricted to neurovascular cells surrounding the condensed dental mesenchymal cells where pSmad1/5 is present. Notably, pSmad1/5 in dental mesenchymal cells form complexes with pSmad3 to prevent canonical BMP signaling. CUT&RUN assays revealed genome-wide co-occupancy of pSmad1/5 and pSmad3, indicating their function as transcriptional regulation units. Integrative analyses demonstrated that this atypical canonical BMP signaling regulates tooth sensory innervation and maintains odontogenic inductive potential in dental mesenchyme, enabling the identification of critical genes for maintaining tooth inductive capability. Our findings elucidate the operating mechanism of atypical canonical BMP signaling in dental mesenchymal cells and clarify how BMP-Smad signaling exerts diverse functions across different cell types, informing future tooth bioengineering strategies.
2025-06-05 | GSE296697 | GEO
Project description:The canonical bone morphogenetic protein (BMP) signaling pathway plays a crucial regulatory role in tooth development by activating Smad proteins to regulate gene expression. We previously identified an atypical canonical BMP signaling in dental mesenchyme that is Smad4-independent but Smad1/5-dependent. This study demonstrates that phosphorylated Smad1/5 (pSmad1/5) and Smad4 regulate distinct gene sets in dental mesenchyme. Real-time monitoring of BMP-Smad transcriptional activity revealed that Smad4-dependent canonical BMP signaling is restricted to neurovascular cells surrounding the condensed dental mesenchymal cells where pSmad1/5 is present. Notably, pSmad1/5 in dental mesenchymal cells form complexes with pSmad3 to prevent canonical BMP signaling. CUT&RUN assays revealed genome-wide co-occupancy of pSmad1/5 and pSmad3, indicating their function as transcriptional regulation units. Integrative analyses demonstrated that this atypical canonical BMP signaling regulates tooth sensory innervation and maintains odontogenic inductive potential in dental mesenchyme, enabling the identification of critical genes for maintaining tooth inductive capability. Our findings elucidate the operating mechanism of atypical canonical BMP signaling in dental mesenchymal cells and clarify how BMP-Smad signaling exerts diverse functions across different cell types, informing future tooth bioengineering strategies.
2025-06-05 | GSE297137 | GEO