Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mkrn3 binds Gnrh1 mRNA to alter subcellular localization of the Gnrh1 precursor protein -Total

ABSTRACT: Loss-of-function mutations in MKRN3 cause central precocious puberty, and our findings show that MKRN3 regulates GnRH production by binding the Gnrh1 3′UTR and altering subcellular localization of its precursor protein through post-transcriptional mechanisms. This function likely involves interactions with RNA-binding proteins such as IGF2BP2 and may extend to other cell types and neuropeptides. We tetsted the changes in the whole proteome upon Mkrn3 overexpression

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Hypothalamus Cell

SUBMITTER: Oded Kleifeld

LAB HEAD: Philippa Melamed

PROVIDER: PXD065897 | Pride | 2026-05-14

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	2024-07-01-decoys-reviewed-contam-UP000000589.fas	Other
	Samples_info.txt	Txt
	Seq78504_Ex480.raw	Raw
	Seq78505_Ex480.raw	Raw
	Seq78506_Ex480.raw	Raw

Items per page:

1 - 5 of 11

Similar Datasets

Mkrn3 binds Gnrh1 mRNA to alter subcellular localization of the Gnrh1 precursor protein - DIA cell fractions

Project description:Loss-of-function mutations in MKRN3 cause central precocious puberty, and our findings show that MKRN3 regulates GnRH production by binding the Gnrh1 3′UTR and altering subcellular localization of its precursor protein through post-transcriptional mechanisms. This function likely involves interactions with RNA-binding proteins such as IGF2BP2 and may extend to other cell types and neuropeptides.

2026-05-14 | PXD065908 | Pride

Mkrn3 binds Gnrh1 mRNA to alter subcellular localization of the Gnrh1 precursor protein - Mkrn3-AP-MS

2026-05-14 | PXD065952 | Pride

RNA binding activity of Mkrn3 directs translation and cellular distribuition of the Gnrh1 precurser protein away from the the site of its processing to the mature GnRH peptide

Project description:Makorin Ring Finger Protein 3 (MKRN3) loss-of-function mutations are the most common genetic cause of central precocious puberty. Its hypothalamic mRNA levels drop leading up to puberty, and it appears to play a crucial repressive role in the central control of pubertal onset. MKRN3 has E3 ubquitin ligase and RNA binding domains, however, its actions in blocking puberty remain unclear, given the diverse actions and targets reported and observed in knockout models. To elucidate its role in GnRH neurons, we generated several high-throughput datasets to examine effects of Mkrn3 on various layers of regulation, including transcription, translation, RNA stability, protein stability, and RNA localization.

2026-05-17 | GSE301395 | GEO

Proteomic Analysis of Mkrn3 WT vs KO mouse brain

Project description:Mutations in the E3 ubiquitin ligase Mkrn3 are associated with precocious puberty in humans. In order to determine the targets of Mkrn3, we performed a TMT-based proteomic analysis of Mkrn3 WT vs KO mouse brains.

2023-07-20 | PXD027220 | Pride

MKRN3 interacting proteins identified by LC-MS/MS

Project description:MKRN3, whose deletion or loss-of-function mutations were genetically associated with human centeral precocious puberty (CPP). To identify the potential substrates for MKRN3, MKRN3 was transfected into HEK293T cells as bait, and its interacting proteins were identified by mass spectrum and functions were extensively studied.

2021-02-22 | PXD022346 | Pride

Effect of MKRN3 deletion on gene expression in hypothalamic neurons derived from human induced pluripotent stem cells (hiPSCs)

Project description:Makorin ring finger protein 3 (MKRN3) was identified as an inhibitor of puberty initiation with the report of loss-of-function mutations in association with central precocious puberty. To investigate the roles and mechanisms of action of MKRN3 within the human hypothalamus, we used hypothalamic neurons derived from human induced pluripotent stem cells (hiPSCs). MKRN3 deletion was introduced into hiPSCs using CRISPR interference (CRISPRi) technology. Three separated hypothalamic differentiation of MKRN3-wildtype hiPSCs (MKRN3-WT I, II and III) and MKRN3-deficient hiPSCs (MKRN3-KO I, II and III) were performed using an established hypothalamic neuron differentiation protocol. To identify hypothalamic targets of MKRN3, we performed comparative transcriptome analysis by RNA sequencing (RNA-seq) of MKRN3-WT and MKRN3-KO hypothalamic neurons after 30 days of differentiation.

2023-06-14 | GSE208722 | GEO

RNA Sequencing for MKRN3 regulating genes identification in GT1-7 cells

Project description:Purpose: The deletion or loss-of-function of MKRN3 were genetically associated with human centeral precocious puberty (CPP).The goal of this study is to explore the global genes expression changes on MKRN3 overexpessing by high-throughput RNA sequencing analysis. Methods: The mRNA profiles of wild-type (WT) and MKRN3 overexpressed GT1-7 cells were generated by deep sequencing, in triplicate, using Illumina GAIIx. The sequence reads that passed quality filters were aligned to mus musculus genomic reference (GRCm38) using HISAT2. Read counts were summarized at the gene level in each sample with HTSeq. Results: Using an optimized data analysis workflow, we mapped about 19 million sequence reads per sample to the mouse genome (build mm10) and identified 13,041 transcripts in the GT1-7 cells of WT and MKRN3. Approximately 6% of the transcripts showed differential expression between the WT and MKRN3 overexpressed cells, with a fold change ≥2 and p-adjust value <0.05. 354 genes were up-regulated by MKRN3, while 395 genes were down-regulated by MKRN3 in this study. Conclusions: Our study is the first effort to explore MKRN3 on gobal genes expression, and hundreds of different expressed genes have been identified.

2021-02-22 | GSE160467 | GEO

RNA-Seq of FGF8 treated neural progenitor cells differentiated from human pluripotent stem cells

Project description:This data would help in understanding the effect of FGF8 in the development of neurons critical for human puberty (GnRH neurons)

2022-07-20 | E-MTAB-11767 | biostudies-arrayexpress