Proteomics

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Proteomic analysis of cervicovaginal fluid for endometriosis biomarker discovery

ABSTRACT: Endometriosis is a gynaecological disease facing significant diagnostic challenges, contributing to an extensive diagnostic delay. Non-invasive diagnostic biomarkers of endometriosis could expedite diagnosis and minimise the reliance on surgery to confirm endometriosis. This pilot study aimed to identify candidate protein biomarkers of endometriosis from cervicovaginal fluid i) collected from consenting people having surgery for suspected endometriosis and ii) sampled via low vaginal swab. Samples from people with (n=20) and without (n=19) endometriosis across were analysed by data independent acquisition mass spectrometry using the Sequential Window Acquisition of All Theoretical Mass Spectra (SWATH)-MS approach. Samples were measured and analysed in two experimental runs (E1 and E2) based on time periods of sample collection. ELISA was performed to validate SWATH-MS findings.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cervicovaginal Fluid

DISEASE(S): Endometriosis

SUBMITTER: Torsten Kleffmann  

LAB HEAD: Claire Henry

PROVIDER: PXD065951 | Pride | 2026-04-06

REPOSITORIES: Pride

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Action DRS
23084_E1_DDA_RunA_F10b.wiff Wiff
23084_E1_DDA_RunA_F10b.wiff.scan Wiff
23084_E1_DDA_RunA_F11b.wiff Wiff
23084_E1_DDA_RunA_F11b.wiff.scan Wiff
23084_E1_DDA_RunA_F1b.wiff Wiff
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Proteomic Analysis of Cervicovaginal Fluid for Diagnostic Endometriosis Biomarker Discovery.

Paterson Emily S J ESJ   Scheck Simon S   Kleffmann Torsten T   Gill Roopi R   Bedford Nicholas N   McDowell Simon S   Girling Jane E JE   Henry Claire E CE  

Proteomics. Clinical applications 20260501 3

<h4>Purpose</h4>Endometriosis is a prevalent inflammatory condition characterised by the presence of endometrial-like tissue outside the uterus and is associated with significant challenges, including diagnostic delays and continued reliance on laparoscopy. Although extensive research has investigated potential biomarkers in various biofluids, none have been validated for clinical use.<h4>Experimental design</h4>This pilot study explored the proteome of cervicovaginal fluid to identify biomarker  ...[more]

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