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Mammalian H4K16ac regulates the spatiotemporal order of genome replication rather than gene expression

ABSTRACT: Histone acetylation is widely assumed to directly instruct gene activation. Among acetylated residues, H4K16ac is one of the most abundant modifications, conserved across all eukaryotes. Here we analyzed histone PTM profiles in MSL KO and HDAC inhibitor-treated cells.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Mammary Gland Epithelial Cell

SUBMITTER: Roberta Noberini

LAB HEAD: Tiziana Bonaldi

PROVIDER: PXD066155 | Pride | 2025-09-29

REPOSITORIES: Pride

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	Experimental_design.txt	Txt
	HF250430_RN_Scaffidi_1.raw	Raw
	HF250430_RN_Scaffidi_10.raw	Raw
	HF250430_RN_Scaffidi_11.raw	Raw
	HF250430_RN_Scaffidi_12.raw	Raw

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Mammalian H4K16ac regulates the spatiotemporal order of genome replication rather than gene expression.

Milan Marta M Runfola Valeria V Patel Manthan M Falbo Lucia L Noberini Roberta R Soriani Chiara C Rodighiero Simona S Bonaldi Tiziana T Costanzo Vincenzo V Pradeepa Madapura M MM Scaffidi Paola P

Nucleic acids research 20250901 18

Histone acetylation is widely assumed to directly instruct gene activation. Among acetylated residues, H4K16ac is one of the most abundant modifications, conserved across all eukaryotes. Despite its established role in X-chromosome hyperactivation in Drosophila, its function in mammalian cells has remained elusive. Here, we show that in human somatic cells, H4K16ac does not substantially affect gene expression, but instead controls the spatiotemporal program of genome replication. By combining a ...[more]

PMID: 40985775

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