Dataset Information

StBPA1 attenuates surface receptor activation and contributes to immune responses against bacteria and oomycete pathogens in potato

ABSTRACT: Plants utilize plasma-membrane-localized pattern recognition receptors (PRRs) to sense and respond to microbial infections. The downstream regulatory components have been studied extensively, but the mechanisms ensuring appropriate immune responses to diverse pathogens remain enigmatic. We report that a core regulatory component named StBPA1 (BINDING PARTNER OF ACD11-1) is a molecular switch that controls both anti-bacterial and anti-oomycete immunity. StBPA1-knockout displays dwarfed growth, enhanced pattern-triggered immunity (PTI), and broad-spectrum resistance to potato bacterial wilt and late blight diseases. StBPA1 negatively regulates the StFLS2-StBAK1/StSOBIR1-StBAK1 immune complex formation and inhibits StFLS2 kinase activity to prevent constitutive immune responses. In turn, StBAK1 specifically phosphorylates StBPA1 at Thr193/Ser195. This modification is enhanced by flg22/INF1 perception and impairs the negative regulatory role of StBPA1, thereby ensuring proper immune signaling. These findings identify an StBPA1-PRR complex regulatory module and highlight inhibitions by StBPA1 as key mechanisms to ensure efficient yet strictly regulated immune responses against different pathogens. Note: StBPA2 in this dataset was later renamed StBPA1 in follow-up experiments and publications.

INSTRUMENT(S):

ORGANISM(S): Solanum Tuberosum (potato)

SUBMITTER: Jie Li

LAB HEAD: Xiaodan Wang

PROVIDER: PXD066169 | Pride | 2025-07-15

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	NegativeContro_StBAK1-KD-D-StBPA.mgf	Mgf
	NegativeContro_StBAK1-KD-D-StBPA.xml	Xml
	NegativeControl_StBAK1-KD-D-StBPA.raw	Raw
	StBAK1-KD-StBPA.mgf	Mgf
	StBAK1-KD-StBPA.raw	Raw

Items per page:

1 - 5 of 7

Similar Datasets

Project description:The earliest stages of development in most metazoans are driven by maternally deposited proteins and mRNAs, with widespread transcriptional activation of the zygotic genome occurring hours after fertilization, at a period known as the maternal-to-zygotic transition (MZT). In Drosophila, the MZT is preceded by the transcription of a small number of genes that initiate sex determination, patterning and other essential developmental processes. The zinc-finger transcription factor Zelda (ZLD) plays a key role in the transcriptional activation of these earliest-expressed genes. To better understand the mechanisms of ZLD activation and the range of its targets, we used chromatin immunoprecipitation coupled with high-throughput sequencing (ChIP-Seq) to map regions bound by ZLD prior to (mitotic cycles 8 and 9), during (mitotic cycles 13 and early 14) and after (late mitotic cycle 14) the MZT. Although only a handful of genes are transcribed prior to mitotic cycle 10, we identified thousands of regions bound by ZLD in cycle 8-9 embryos, most of which remain bound through mitotic cycle 14. As expected, these ZLD-bound regions include the promoters and enhancers of the small subset of genes transcribed at this early stage. However we also observed ZLD bound at cycle 8-9 to the promoters of a large fraction of the several thousand genes whose first transcription does not occur until roughly an hour and four mitotic cycles later. These early ZLD-bound regions include virtually all of the thousands of known and presumed enhancers bound at cycle 14 by the transcription factors that regulate patterned gene activation during the MZT. The association between early ZLD binding and MZT activity is so strong that ZLD binding alone can be used to identify active promoters and regulatory sequences with high specificity and selectivity. This strong early association of ZLD with regions not active until the MZT suggests that ZLD is not only required for the earliest wave of transcription, but also plays a major role in activating the genome at the MZT. Genome-wide mapping of Zelda in wild-type Drosophila melanogaster embryos prior (mitotic cycles 8-9), during (cycles 13-14), and after (late cycle 14) maternal-to-zygotic transition

Dataset Information

StBPA1 attenuates surface receptor activation and contributes to immune responses against bacteria and oomycete pathogens in potato

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets