Proteome Profiling in the Rat Dorsal Striatum Synaptoneurosome Following Methamphetamine Exposure

ABSTRACT: We performed discovery-based proteomic profiling of synaptoneurosomes (SNs), subcellular fractions of brain tissue containing both pre- and post-synaptic components, isolated from the dorsal striatum of rats following methamphetamine (Meth) administration to investigate how methamphetamine exposure alters the synaptic proteome. Using tandem mass tag (TMT)-based multiplexed quantification with high-resolution mass spectrometry (HRMS), we identified 6094 proteins and quantified 4583 in 3 out 5 biological replicates. Comparative analysis between Meth-treated and control rats revealed differential regulation for 147 proteins. These proteins were enriched in synaptic, mitochondrial, and proteasomal pathways.

INSTRUMENT(S):

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Brain

SUBMITTER: Peter Nemes  

LAB HEAD: Peter Nemes

PROVIDER: PXD066175 | Pride | 2025-11-19

REPOSITORIES: Pride