Project description:For PSMD5 over expression experiment, PSMB4-tagged cells were transfected in either 6-well plates (Cat. No. 3506; Costar) or 10 cm dishes using 1 µg or 5 µg of HA-PSMD5 or HA-PSMD5ΔCT, respectively. All the transfections were performed with Lipofectamine 3000 (Cat. No. L3000015; Thermo Fisher Scientific) in Opti-MEM reduced serum medium (Cat. No. 31985070; Life Technologies), following the manufacturer’s protocol. Cells were incubated at 37 °C in a humidified atmosphere containing 5 % CO₂ for 48 h before harvesting.
Project description:All raw and processed Pulldown-ID mass-spectrometry datasets used in 'Atlas of the proteasome interactome by in situ structural biology': Pulldown ID-1 (non-crosslinked WT vs RPN1 tag); Pulldown ID-2 (crosslinked WT vs RPN1 tag); Pulldown ID-3 (non-crosslinked WT vs RPN11 tag); Pulldown ID-4 (crosslinked WT vs RPN11 tag); Pulldown ID-5 (non-crosslinked WT vs β4 tag); and Pulldown ID-6 (crosslinked WT vs β4 tag).
Project description:All raw and processed Pulldown-ID mass-spectrometry datasets used in 'Atlas of the proteasome interactome by in situ structural biology': Pulldown ID-1 (non-crosslinked WT vs RPN1 tag); Pulldown ID-2 (crosslinked WT vs RPN1 tag); Pulldown ID-3 (non-crosslinked WT vs RPN11 tag); Pulldown ID-4 (crosslinked WT vs RPN11 tag); Pulldown ID-5 (non-crosslinked WT vs β4 tag); and Pulldown ID-6 (crosslinked WT vs β4 tag).
Project description:All raw and processed Pulldown-ID mass-spectrometry datasets used in 'Atlas of the proteasome interactome by in situ structural biology': Pulldown ID-1 (non-crosslinked WT vs RPN1 tag); Pulldown ID-2 (crosslinked WT vs RPN1 tag); Pulldown ID-3 (non-crosslinked WT vs RPN11 tag); Pulldown ID-4 (crosslinked WT vs RPN11 tag); Pulldown ID-5 (non-crosslinked WT vs β4 tag); and Pulldown ID-6 (crosslinked WT vs β4 tag).
Project description:All raw and processed Pulldown-ID mass-spectrometry datasets used in 'Atlas of the proteasome interactome by in situ structural biology': Pulldown ID-1 (non-crosslinked WT vs RPN1 tag); Pulldown ID-2 (crosslinked WT vs RPN1 tag); Pulldown ID-3 (non-crosslinked WT vs RPN11 tag); Pulldown ID-4 (crosslinked WT vs RPN11 tag); Pulldown ID-5 (non-crosslinked WT vs β4 tag); and Pulldown ID-6 (crosslinked WT vs β4 tag).
Project description:All raw and processed Pulldown-ID mass-spectrometry datasets used in 'Atlas of the proteasome interactome by in situ structural biology': Pulldown ID-1 (non-crosslinked WT vs RPN1 tag); Pulldown ID-2 (crosslinked WT vs RPN1 tag); Pulldown ID-3 (non-crosslinked WT vs RPN11 tag); Pulldown ID-4 (crosslinked WT vs RPN11 tag); Pulldown ID-5 (non-crosslinked WT vs β4 tag); and Pulldown ID-6 (crosslinked WT vs β4 tag).
Project description:All raw and processed Pulldown-ID mass-spectrometry datasets used in 'Atlas of the proteasome interactome by in situ structural biology': Pulldown ID-1 (non-crosslinked WT vs RPN1 tag); Pulldown ID-2 (crosslinked WT vs RPN1 tag); Pulldown ID-3 (non-crosslinked WT vs RPN11 tag); Pulldown ID-4 (crosslinked WT vs RPN11 tag); Pulldown ID-5 (non-crosslinked WT vs β4 tag); and Pulldown ID-6 (crosslinked WT vs β4 tag).
Project description:For PSMD5 over expression experiment, RPN1-tagged cells were transfected in either 6-well plates (Cat. No. 3506; Costar) or 10 cm dishes using 1 µg or 5 µg of HA-PSMD5, respectively. All the transfections were performed with Lipofectamine 3000 (Cat. No. L3000015; Thermo Fisher Scientific) in Opti-MEM reduced serum medium (Cat. No. 31985070; Life Technologies), following the manufacturer’s protocol. Cells were incubated at 37 °C in a humidified atmosphere containing 5 % CO₂ for 48 h before harvesting.
Project description:Identification of the binding interface between HttExon1Q48 and the molecular chaperone DNAJB1. The proteins were incubated and cross-linked in vitro and the binding interface was mapped to the hinge region between CTDI and CTDII of DNAJB1 and the proline rich domain of HTTEXon1Q48.