Dataset Information

Comprehensive developmental somatic proteome atlas of Haemonchus contortus underpinned by a chromosome-scale genome and deep tandem mass spectrometry

ABSTRACT: Haemonchus contortus is a highly pathogenic, blood-feeding nematode that causes widespread disease and significant economic loss in livestock worldwide. Previous proteomic studies were constrained by incomplete genomic resources and limited analytical sensitivity, impeding comprehensive profiling across life stages. In this study, we integrated advanced tandem mass spectrometry with a chromosome-scale genome assembly of the Haecon-5 strain to construct the most detailed somatic proteome of H. contortus to date. We identified and quantified 7,002 proteins across five key developmental stages and sexes (i.e. egg, L3, L4, adult female and adult male), tripling the number identified in previous studies. Comparative analyses revealed pronounced stage-specific expression and functional specialisation, with parasitic stages enriched in proteins linked to metabolism, cellular function and environmental sensing. Fifteen proteins associated with the hypoxia-inducible factor 1 (HIF-1) signalling pathway were upregulated in parasitic stages, suggesting a role in adaptation to hypoxia. Additionally, 150 proteases implicated in haemoglobin degradation were characterised, and functional assays confirmed markedly elevated haemoglobinolytic activity in blood-feeding stages. These findings offer key insights into H. contortus development and parasitism, and establish a high-resolution proteomic framework to underpin fundamental biological studies and enable the discovery of molecular targets for novel interventions against this and related nematodes.

INSTRUMENT(S):

ORGANISM(S): Haemonchus Contortus (barber Pole Worm)

TISSUE(S): Whole Body

SUBMITTER: Tao Wang

LAB HEAD: Robin Beat Gasser

PROVIDER: PXD066296 | Pride | 2025-09-11

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	250131_Repeat_TW-10170_DDA_1.raw	Raw
	250131_Repeat_TW-10170_DDA_2.raw	Raw
	250131_Repeat_TW-10170_DDA_3.raw	Raw
	250131_Repeat_TW-10170_DDA_4.raw	Raw
	250131_Repeat_TW-10170_DDA_5.raw	Raw

Items per page:

1 - 5 of 33

Similar Datasets

Project description:Infections and diseases caused by parasitic nematodes have a major adverse impact on the health and productivity of animals and humans worldwide. The control of these parasites often relies heavily on the treatment with commercially available chemical compounds (anthelmintics). However, the excessive or uncontrolled use of these compounds in livestock animals has led to major challenges linked to drug resistance in nematodes. Therefore, there is a need to develop new anthelmintics with novel mechanism(s) of action. Recently, we identified a small molecule, KM08948, with nematocidal activity against the free-living model organism Caenorhabditis elegans. Here, we evaluated KM08948’s potential as an anthelmintic in a structure-activity relationship (SAR) study in C. elegans and in the highly pathogenic, blood-feeding Haemonchus contortus (barber’s pole worm), and explored the compound-target relationship using thermal proteome profiling (TPP). First, we synthesised and tested a series of 25 KM08948 analogues for nematocidal activity in both H. contortus (larvae and adults) and C. elegans (young adults), establishing a preliminary nematocidal pharmacophore for both species. We identified several compounds with marked activity against either H. contortus or C. elegans which had greater efficacy than KM08948, and found a significant divergence in compound bioactivity between these two nematode species. We also identified a KM08948 analogue, 25, that moderately inhibited the motility of adult female H. contortus in vitro. Subsequently, we inferred three H. contortus proteins (HCON_00134350, HCON_00021470 and HCON_00099760) and five C. elegans proteins (F30A10.9, F15B9.8, B0361.6, DNC-4 and UNC-11) that interacted directly with KM08948; however, no conserved protein target was shared between the two nematode species. Future work aims to extend the SAR investigation in these and other parasitic nematode species, and validate individual proteins identified here as targets of KM08948. Overall, the present study describes an evaluation of this anthelmintic candidate and highlights some challenges associated with early anthelmintic discovery.

Dataset Information

Comprehensive developmental somatic proteome atlas of Haemonchus contortus underpinned by a chromosome-scale genome and deep tandem mass spectrometry

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets