Proteomics

Dataset Information

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MCF7 treated with abemaciclib nuclear Rb IP LC-MSMS


ABSTRACT: The retinoblastoma protein (Rb) is a tumour suppressor best known for repressing E2F transcription factors and halting cell cycle progression1. In hormone receptor-positive (HR+) breast cancer, CDK4/6 inhibitors activate Rb by preventing its phosphorylation, forming a key component of current endocrine therapy regimens2. How pharmacologically activated Rb remodels chromatin and influences transcriptional networks beyond cell cycle arrest remains poorly understood. We show that CDK4/6 inhibition induces widespread redistribution of hypo-phosphorylated Rb to both promoters and enhancers in cancer cells. While Rb predictably binds to cell cycle gene promoters to repress transcription, at other sites it unexpectedly promotes expression of oestrogen-responsive genes by integrating into oestrogen receptor (ER)-rich transcriptional hubs. In this dataset, we investigate chromatin modifiers that can interact with Rb in the nucleus with CDK4/6 inhibitor treatment in the ER+ breast cancer cell line MCF7.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Malignant Cell, Breast

DISEASE(S): Breast Cancer

SUBMITTER: April C. Watt  

LAB HEAD: Shom Goel

PROVIDER: PXD066496 | Pride | 2026-06-23

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20250214_094410_Abemaciclib_RB1_vs_Abemaciclib_IgG.sne Other
241217_MT-10045_04.raw Raw
241217_MT-10045_05.raw Raw
241217_MT-10045_06.raw Raw
241217_MT-10045_10.raw Raw
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