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RPN1 pulldown ID with PSMD5 overexpression from 'Integrated structural biology and in situ crosslinking reveals UBE3C, TXNL1, and PSMD5 at proteasome complexes'

ABSTRACT: For PSMD5 over expression experiment, RPN1-tagged cells were transfected in either 6-well plates (Cat. No. 3506; Costar) or 10 cm dishes using 1 µg or 5 µg of HA-PSMD5, respectively. All the transfections were performed with Lipofectamine 3000 (Cat. No. L3000015; Thermo Fisher Scientific) in Opti-MEM reduced serum medium (Cat. No. 31985070; Life Technologies), following the manufacturer’s protocol. Cells were incubated at 37 °C in a humidified atmosphere containing 5 % CO₂ for 48 h before harvesting.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Kitaik Lee

LAB HEAD: Francis J.O'Reilly

PROVIDER: PXD066535 | Pride | 2026-07-16

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	E250325_01_KL_OX_PSMD5_pulldownID_Control_01_inj1.raw	Raw
	E250325_02_KL_OX_PSMD5_pulldownID_Control_01_inj2.raw	Raw
	E250325_04_KL_OX_PSMD5_pulldownID_Control_02_inj1.raw	Raw
	E250325_05_KL_OX_PSMD5_pulldownID_Control_02_inj2.raw	Raw
	E250325_07_KL_OX_PSMD5_pulldownID_Control_03_inj1.raw	Raw

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