Proteomics

Dataset Information

0

Comparative Muscle Proteomics in Organic and Conventional Broiler Chickens: Uncovering Biomarkers of Stress and Meat Quality

ABSTRACT: This study investigates the impact of stress on muscle physiology and meat quality in broiler chickens by comparing protein expression profiles between organic and conventional farming systems using label-free quantitative (LFQ) proteomics. Muscle samples were analyzed via nanoLC-ESI-MS/MS coupled with comprehensive bioinformatics to identify differences in protein abundance associated with rearing conditions.A total of 7,221 proteins were identified, with 1,645 proteins upregulated and 1,612 downregulated in organic chickens compared to conventional ones. Functional analyses including Gene Ontology (GO) and STRING network analyses revealed that proteins upregulated in organic chickens were predominantly involved in oxygen transport, oxygen binding, and muscle structural organization, indicating enhanced oxygen metabolism and muscle development consistent with improved animal welfare. Conversely, proteins related to ribosomal function and RNA binding were enriched in conventional chickens, suggesting stress-related alterations in protein synthesis. KEGG pathway analysis showed significant enrichment of carbon metabolism, amino acid biosynthesis, nitrogen metabolism, and the tricarboxylic acid (TCA) cycle pathways in organic chickens, while glycolysis, gluconeogenesis, and ribosomal pathways were downregulated. Key differentially expressed proteins identified as potential biomarkers distinguishing organic from conventional meat include downregulated PGM1, AMPD1, LDHA, ENO3, and PKLR, and upregulated COL1A1, COL1A2, TTN, TPM2, CA3, MB, HSPB1, ACO2, ACAA2, and TF. These proteins are involved in muscle structure and energy metabolism and may serve as indicators of meat quality linked to stress and welfare conditions. Overall, this proteomic analysis provides novel insights into how stress modulates the muscle proteome in broiler chickens and supports the adoption of welfare-focused organic poultry production practices to improve meat quality.

INSTRUMENT(S):

ORGANISM(S): Gallus Gallus (chicken)

SUBMITTER: Ivanka Krasteva  

LAB HEAD: Mirella Luciani

PROVIDER: PXD066589 | Pride | 2025-12-29

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
Chicken_Organic_1a.mzML Mzml
Chicken_Organic_1a.mzid Mzid
Chicken_Organic_1a.raw Raw
Chicken_Organic_1b.mzML Mzml
Chicken_Organic_1b.mzid Mzid
Items per page:
1 - 5 of 19
altmetric image

Publications

Muscle proteomics reveals biomarkers of stress and meat quality in organic versus conventional broiler chickens.

Luciani M M   Schirone Maria M   Krasteva I I   Pancrazio C Di CD   Sacchini F F   Maggetti M M   Perletta F F   D'Alterio N N   Paparella A A   Vulpiani M Podaliri MP   Iannetti L L  

Poultry science 20251126 1

Animal welfare is a crucial factor in animal production, given its impact on ethics, consumer choices, and meat quality. Stress markedly influences poultry physiology and meat quality by affecting muscle biochemistry and key attributes such as tenderness, flavor, and color. This study employed a label-free quantitative (LFQ) proteomics approach using nLC-ESI-MS/MS combined with bioinformatics to compare muscle protein expression in broiler chickens raised under organic versus conventional produc  ...[more]

Similar Datasets

Transcriptomics Identification of candidate genes associated with breast muscle of broilers exposed to atmospheric ammonia using RNA sequencing
2017-10-14 | GSE104941 | GEO
Proteomics Effect of an immune challenge and feed supplements on broiler chicken individual breast muscle protein synthesis
2024-04-04 | PXD044325 | Pride
Proteomics Proteomics Analysis of Bacterial Chondronecrosis with Osteomyelitis in Broiler Chickens
2023-05-10 | PXD029085 | Pride
Proteomics Enhanced Media Optimize Bovine Myogenesis in 2D and 3D Models for Cultivated Meat Applications
2025-07-24 | PXD051019 | Pride
Transcriptomics Enhanced Media Optimize Bovine Myogenesis in 2D and 3D Models for Cultivated Meat Applications [in vitro scRNA-Seq]
2025-07-25 | GSE262757 | GEO
Transcriptomics Enhanced Media Optimize Bovine Myogenesis in 2D and 3D Models for Cultivated Meat Applications [RNA-Seq]
2025-07-25 | GSE262675 | GEO
Transcriptomics Enhanced Media Optimize Bovine Myogenesis in 2D and 3D Models for Cultivated Meat Applications [in vivo scRNA-Seq]
2025-07-25 | GSE290556 | GEO
Transcriptomics Transcriptome profiling of the liver and pectoralis major muscle in slow-growing chickens under an organic raising system
2023-12-01 | GSE231912 | GEO
Transcriptomics Differential gene expression in the bone marrow of 60 week old layer and broiler chickens
2007-01-01 | GSE6270 | GEO
Transcriptomics Differential gene expression in the bone marrow of 15 week old layer and broiler chickens
2007-01-01 | GSE6268 | GEO