HeLa cell protein interactome of polyamine affinity-based probes (probe-modified peptides)

ABSTRACT: HeLa cells were incubated with affinity-based probes designed as synthetic analogs of endogenous metabolites: putrescine, spermidine, and spermine. Probe-protein interactions were captured in situ using UV-induced crosslinking. After cell lysis, the probe–protein conjugates were subjected to bioorthogonal ligation with a biotinylation reagent, followed by tryptic digestion and affinity purification of the probe-labeled peptides, which were then analyzed by LC-MS/MS. The dataset was obtained with the support of the National Science Centre, Poland grant Sonata Bis (2020/38/E/ST4/00250).

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hela Cell

SUBMITTER: Remigiusz Serwa  

LAB HEAD: Remigiusz Serwa

PROVIDER: PXD066655 | Pride | 2025-09-22

REPOSITORIES: Pride