Dataset Information

Comparative protein profiles in acorns collected from elevated CO2 and ambient CO2.

ABSTRACT: Using data from acorns produced by mature oak trees in the eighth year of elevated CO2 (eCO₂), we present evidence that similar effects occur in long-established forests, with negative consequences for seed quality, that impact herbivore nutrition and health. The analysis of acorns from the near-200-year-old oak trees at the Free Air Carbon dioxide (FACE) facility at the Birmingham Institute for Forest Research (BIFoR) revealed that growth under eCO₂ increased the phytate content but decreased the protein content of acorns. Additionally the analysis of protin profiles showed significant differnves in protein abundances in both typoe of samples.

INSTRUMENT(S):

ORGANISM(S): Quercus Robur

TISSUE(S): Seed

SUBMITTER: Barbara Karpinska

LAB HEAD: Barbara Karpinska

PROVIDER: PXD066721 | Pride | 2025-11-17

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

Items per page:

1 - 5 of 15

Publications

Elevated atmospheric CO2-induced reprogramming leads to decreased seed protein and nutritional quality in forest trees.

Karpinska Barbara B Sanchez-Lucas Rosa R Plackett Andrew A MacKenzie A Rob AR Foyer Christine Helen CH

Plant physiology 20251001 3

While the global increases in atmospheric CO2 levels have had a beneficial effect on plant growth, the negative impacts of this CO2 fertilization effect on seed quality are often overlooked. Using data from acorns produced by mature oak (Quercus robur) trees in the eighth year of eCO2, we present evidence of negative consequences for seed quality. The acorns produced by the near-200-yr-old oak trees under eCO2 at the free air carbon dioxide enrichment facility at the Birmingham Institute for For ...[more]

PMID: 41026902

Similar Datasets

Project description:• Reference to published study making use of this data: • Cseke LJ, Tsai C-J, Rogers A, Nelsen MP, White HL, Karnosky DF, Podila GK. (2009) Transcriptomic comparison in the leaves of two aspen genotypes having similar carbon assimilation rates but different allocation patterns under elevated CO2. New Phytologist. submitted. • This study compared the leaf transcription profiles, physiological characteristics, and primary metabolites of two Populus tremuloides genotypes (clones 216 and 271) known to differ in their responses to long-term elevated [CO2] (e[CO2]) at the Aspen FACE site near Rhinelander, WI. • Physiological responses of these clones are similar in photosynthesis, stomatal conductance, and leaf area index under e[CO2] yet very different in growth enhancement (0-10% in clone 216; 40-50% in clone 271). While few genes responded to long-term exposure to e[CO2], the transcriptional activity of leaf e[CO2]-responsive genes was distinctly different between the clones, differentially impacting multiple pathways during both early and late growing seasons. • Analysis of transcript abundance and carbon/nitrogen biochemistry suggests that the CO2-responsive clone (271) partitions C into pathways associated with active defense/response to stress, carbohydrate/starch biosynthesis and subsequent growth. The CO2-unresponsive clone (216) partitions C into pathways associated with passive defense (e.g. lignin, phenylpropanoid) and cell wall thickening. • This study indicates that there is significant variation in expression patterns between different tree genotypes in response to long-term exposure to e[CO2]. Consequently, future efforts to improve productivity or other advantageous traits for carbon sequestration should include an examination of genetic variability in CO2 responsiveness. Keywords: Tree genotype comparison under elevated [CO2] 24 two-channel arrays, directly comparing RNA from trees grown in the ambient (control) [CO2] to RNA derived from the same genotype grown under e[CO2]. For each of 4 experimental conditions (clone 216 early season; clone 271 early season; clone 216 late season; clone 271 late season), three independent biological replicates derived from trees grown in three independent replicate FACE rings were used. In addition, each clone and time point included dye swap reciprocal two-color experiments for each biological replicate. Thus, six data points per cDNA are included (three biological replicates with two technical replicates each).

			Action	DRS
	080324_BK_1H.raw	Raw
	080324_BK_1N.raw	Raw
	080324_BK_2H.raw	Raw
	080324_BK_2N.raw	Raw
	080324_BK_3H.raw	Raw