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Suboptimal codon pairs trigger ribosome collisions and cellular quality control responses in tRNA modification mutants

ABSTRACT: Validation of activation of RQC in U34 tRNA modification mutants by identification of ubiquitination of ribosomal proteins uS3 and uS10.

INSTRUMENT(S):

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Manfred Heller

LAB HEAD: Sebastian Leidel

PROVIDER: PXD066757 | Pride | 2025-12-29

REPOSITORIES: Pride

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			Action	DRS
	20220707_D1_EC_i01.raw	Raw
	20220707_D1_EC_i02.raw	Raw
	20220707_D2_EC_i01.raw	Raw
	20220707_D2_EC_i02.raw	Raw
	20220707_D3_EC_i01.raw	Raw

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Suboptimal codon pairs trigger ribosome collisions and cellular quality control responses in tRNA modification mutants.

Wu Jie J Eggers Cristian C Sin Olga O Koziej Łukasz Ł Mancilla Hector H Mollet Fabienne F Schöler Hans R HR Drexler Hannes C A HCA Ranff Tristan T Fufezan Christian C Kraft Claudine C Glatt Sebastian S Bruder Jan M JM Leidel Sebastian A SA

Nucleic acids research 20251101 22

Transfer RNA (tRNA) modifications tune translation rates and codon optimality, thereby optimizing co-translational protein folding. However, the mechanisms by which tRNA modifications modulate codon optimality and trigger phenotypes remain unclear. Here, we show that ribosomes stall at specific modification-dependent codon pairs in wobble uridine modification (U34) mutants. This triggers ribosome collisions and a coordinated hierarchical response of cellular quality control pathways. High-resolu ...[more]

PMID: 41429421

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