Stereo-random Oligonucleotides Enable Efficient Recruitment of ADAR in vitro and in vivo

ABSTRACT: We used our previously published isASO-ID protocol to identify binders for two different adenosine deaminases acting on RNA- (ADAR-) recruiting antisense oligonucleotides (ONs). The ONs differed in their phosphorothioate content and their RNA editing potency and efficiency through the recruitment of endogenous ADAR. We aimed to get a first insight into the protein interactions of this new ON class since ON toxicity is largely defined by the protein interactions. The results will help to understand class-specific mechanisms for ON toxicity and thus to design safer ON drugs.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell

SUBMITTER: Mirita Franz-Wachtel  

LAB HEAD: Thorsten Stafforst

PROVIDER: PXD066763 | Pride | 2025-07-31

REPOSITORIES: Pride