Site-specific N-glycoproteomic analysis of extracellular vesicles from seminal plasma for the molecular typing of azoospermia

ABSTRACT: This dataset contains mass spectrometry raw files and identification results from intact N-glycoproteomic analysis of extracellular vesicles (EVs) isolated from human seminal plasma. Intact N-glycopeptides were enriched and analyzed using an Orbitrap Fusion Lumos mass spectrometer coupled to an Easy-nLC 1200 system. The uploaded dataset includes: (1) Data-dependent acquisition (DDA) LC-MS/MS files acquired via stepped collision energy higher-energy collision dissociation (StepHCD) across discovery cohort samples; and (2) StepHCD-based parallel reaction monitoring (PRM) targeted LC-MS/MS files across validation cohort samples. Raw data files were searched against the human UniProt reviewed database using Glyco-Decipher software with a 1% false discovery rate (FDR).

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Mingyang Li  

LAB HEAD: Xuejiang Guo

PROVIDER: PXD066843 | Pride | 2026-08-15

REPOSITORIES: Pride