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Proteome of DiFi EVs, exomeres and supermeres purified by ultracentrifugation and FPLC

ABSTRACT: The cell seretome is diverse and contains extracellular secreted vesicles (EVs) as well as non-vesicular extracellular nanoparticles (NVEPs) called exomeres and supermeres, all of which carry different kinds of cell signaling cargos. We have developed new techniques to purify exomeres and supermeres by ultracentrifugation (UC) and by fast protein liquid chromatography (FPLC) from cell conditioned media and from blood plasma. We utilized a proteomics approach to compare UC and FPLC methods with standard UC and density gradient methods of isolating EVs and new methods of isolating NVEPs. We acquired LC-MS/MS data from conditioned media using standard 2D plastic and hollow fiber 3D bioreactor production from a CRC cell line, DiFi, using all these methods and growth culture conditions.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Colon

DISEASE(S): Colon Cancer

SUBMITTER: Kristie Lindsey Rose

LAB HEAD: Kristie Lindsey Rose

PROVIDER: PXD066872 | Pride | 2025-09-11

REPOSITORIES: Pride

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			Action	DRS
	Sample_labels_6355.xlsx	Xlsx
	Samples_Protein_Report_Proj6355.xlsx	Xlsx
	SpectrumReport_Proj6355.xlsx	Xlsx
	pEX2_pp_6352_20231110_S10_2CW_Weaver_1pt3uL_10.raw	Raw
	pEX2_pp_6352_20231110_S11_3CW_Weaver_1uL_11.raw	Raw

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