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Multi-organ proteomic atlas of obesity regression in male mice

ABSTRACT: Obesity drives systemic metabolic dysfunction, yet how the body adapts and recovers at a system-wide level remains unclear. Here we generate a multi-tissue proteomic atlas of diet-induced obesity and its regression in mice. Using a standardized, semi-automated sample preparation workflow, we quantify 12,936 unique proteins across 15 organs and 4 timepoints. We found proteomic changes to be highly tissue-dependent with a small number of proteins displaying shared changes across multiple tissues. While proteomes of most tissues revert to lean levels after weight loss, white adipose tissue retains strong phagocytic and inflammatory responses, and brain, kidney, bone, thymus, and spleen display delayed obesity-induced alterations. We map the adipose tissue-specific immune regulators using ligand-target inference and show reduced expression of proteasomal subunits in brown adipose tissue, resulting in stalled ubiquitin turnover. Finally, we make all datasets open access and create an interactive Reactome-based webtool to facilitate further community-driven discovery.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Spleen, Heart, Brain, Skeletal Muscle, Duodenum, Brown Adipose Tissue, Liver, Whole Body, Kidney, Thymus, Jejunum, White Adipose Tissue, Epididymal White Adipose Tissue, Blood Serum, Bone, Pancreas

SUBMITTER: Felix Boel

LAB HEAD: Blagoy Blagoev

PROVIDER: PXD066875 | Pride | 2026-07-24

REPOSITORIES: Pride

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			Action	DRS
	05092023_FP_MmObesityRegression_Bone_3693.raw	Raw
	05092023_FP_MmObesityRegression_Bone_3695.raw	Raw
	05092023_FP_MmObesityRegression_Bone_3699.raw	Raw
	05092023_FP_MmObesityRegression_Bone_3700.raw	Raw
	05092023_FP_MmObesityRegression_Bone_3701.raw	Raw

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