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Fine-Tuning of Label-Free Single-Cell Proteomics Workflows

ABSTRACT: This project aims to compare three distinct workflows using different sample collection/preparation plate supports (EVO96 oil-free, LF48 oil-based and LF48 oil-free, a streamlined automated sample resuspension and direct injection protocol). Then, digestion efficiency using a range of protein/enzyme ratios (1:1; 10:1; 20:1; 50:1) to maximize peptide recovery was evaluated. Finally, the chromatographic setup was refined to determine the best compromise between throughput and robustness.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Cervix Carcinoma

SUBMITTER: Pauline Perdu-Alloy

LAB HEAD: Christine CARAPITO

PROVIDER: PXD067019 | Pride | 2025-12-12

REPOSITORIES: Pride

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			Action	DRS
	25cm.zip	Other
	5cm.zip	Other
	Human_pSP_CMO_20190213_iRT_validated.fasta	Fasta
	Search-report_LF48oilfree.ratiodigest-withMBR.1missclv.libfree_pg_pr.zip	Other
	Search_report-EVO96.ratiodigest10-1-withMBR.1missclv.libfree_pg_pr.zip	Other

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