Outer membrane and Whole cell proteome Analysis of tamA, tamB and tamAB knockout mutants compared to the wildtype

ABSTRACT: The Translocation and Assembly Module (TAM), composed of TamA and TamB, facilitates the insertion of some β-barrel proteins into the OM of Escherichia coli and Klebsiella pneumoniae, and has also been implicated in lipid homeostasis. However, its role in Pseudomonas aeruginosa remains mostly uncharacterized. To investigate TAM’s function and drug target potential in P. aeruginosa, we generated both single gene knockouts and the tamAB double knockout and isolated outer membrane proteins (OMP) and whole cell lysate (WCP) and run mass spectrometry.

INSTRUMENT(S):

ORGANISM(S): Pseudomonas Aeruginosa Pa14

TISSUE(S): Cell Culture

SUBMITTER: David Boocock  

LAB HEAD: David Boocock

PROVIDER: PXD067041 | Pride | 2025-09-16

REPOSITORIES: Pride