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Effect of heat-shock on total and poly-ubiquitylated proteomes of human A549 lung adeno-carcinoma cells in different proliferation states

ABSTRACT: Cellular stress responses are crucial for survival in sub-optimal conditions, and almost invar-iably involve proteome-wide alterations at the levels of protein abundance and post-translational modification with poly-ubiquitin chains (poly-ubiquitylation). However, most of our understanding of human cell stress responses at the protein level stems from experiments in rapidly-dividing cell culture models. Here, we used the A549 human diploid lung adenocarci-noma cell line (epithelial, alveolar type-2) in proliferating or bleomycin-induced senescent states, to explore their responses to a severe heat-shock (44 degrees Celsius for 2 hours) at the total and poly-ubiquitylated proteome levels

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: Harvey Johnston

LAB HEAD: Rahul Samant

PROVIDER: PXD067153 | Pride | 2025-10-08

REPOSITORIES: Pride

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			Action	DRS
	File_description_summary.xlsx	Xlsx
	Ref1023_TMT18_A549_44.msf	Msf
	Ref_1023-10_FAIMS_RTS_MS3.raw	Raw
	Ref_1023-11_FAIMS_RTS_MS3.raw	Raw
	Ref_1023-12_FAIMS_RTS_MS3.raw	Raw

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