Dataset Information

Bee lymphocyte surfaceome analysis

ABSTRACT: This study maps the surfaceome of Apis mellifera hemocytes, the protagonist cells in honey bee cellular immunity. The surfaceome, proteins expressed at the cell surface, is crucial as it determines how cells interact with their microenvironment. Through a combination of proteomic and transcriptomic analyses, 1101 genes encoding cell surface proteins were identified, revealing a high level of diversity.

INSTRUMENT(S):

ORGANISM(S): Apis Mellifera (honeybee)

TISSUE(S): Lymphocyte

SUBMITTER: Bart Devreese

LAB HEAD: Bart Devreese

PROVIDER: PXD067259 | Pride | 2025-09-13

REPOSITORIES: Pride

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Source:

			Action	DRS
	F010806b_1p_uPAC50Viper4_trapPM9_EasySpray_60min_PRC-6235_B_2.raw	Raw
	F010808b_1p_uPAC50Viper4_trapPM9_EasySpray_60min_PRC-6235_C_3.raw	Raw
	F010810b_1p_uPAC50Viper4_trapPM9_EasySpray_60min_PRC-6235_D_4.raw	Raw
	F010812b_1p_uPAC50Viper4_trapPM9_EasySpray_60min_PRC-6235_E_5.raw	Raw
	F010814b_1p_uPAC50Viper4_trapPM9_EasySpray_60min_PRC-6235_F_6.raw	Raw

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Project description:The squid, Euprymna scolopes, and the luminescent bacterium, Vibrio fischeri, form a highly specific beneficial light organ symbiosis. Not only does the host have to select V. fischeri from the environment, but it must also prevent subsequent colonization by non-symbiotic microorganisms. Host macrophage-like hemocytes are believed to play a role in mediating the symbiosis with V. fischeri. Previous studies have shown that the colonization state of the light organ influences the host’s hemocyte response to the symbiont. To further understand the molecular mechanisms behind this process, two quantitative mass spectrometry-based proteomic techniques, isobaric tags for relative and absolute quantification (iTRAQ) and label-free spectral counting, were used to compare and quantify the adult hemocyte proteomes from colonized (sym) and uncolonized (antibiotic-treated/cured) squid. Overall, iTRAQ allowed for the quantification of 1,024 proteins with 2 or more peptides. Thirty-seven unique proteins were determined to be significantly different between sym and cured hemocytes (p-value < 0.05), with 20 more abundant proteins and 17 less abundant in sym hemocytes. The label-free approach resulted in 1,241 proteins that were identified in all replicates. Of 185 unique proteins present at significantly different amounts in sym hemocytes (as determined by spectral counting), 92 were more abundant and 93 were less abundant. Comparisons between iTRAQ and spectral counting revealed that 30 of the 37 proteins quantified by iTRAQ exhibited similar trends identified by the label-free method. Both proteomic techniques mutually identified 16 proteins that were significantly different between the two groups of hemocytes (p-value <0.05). The presence of V. fischeri in the host light organ influenced the abundance of proteins associated with the cytoskeleton, adhesion, lysosomes, proteolysis, and the innate immune response. These data provide evidence that colonization by V. fischeri alters the hemocyte proteome and reveals proteins that may be important for maintaining host-symbiont specificity.

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Bee lymphocyte surfaceome analysis

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