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Annexin A2 stabilizes the endoplasmic reticulum and actin cytoskeleton and regulates formation of reovirus factories

ABSTRACT: Early in infection, mammalian orthoreoviruses (reoviruses) build neo-organelles called viral factories (VFs). These structures incorporate fragments of endoplasmic reticulum (ER) and serve as sites of viral genome replication and particle assembly. Two reovirus nonstructural proteins, σNS and µNS, remodel the ER to produce the vesicles and tubules that form the VF matrix. Using co-immunoprecipitation assays followed by mass spectrometry, we identified annexin A2 (ANXA2), which binds actin and cellular membranes, as a host factor that interacts with reovirus nonstructural proteins. In the absence of ANXA2, VF formation was accelerated in the early phases of reovirus infection, and yields of reovirus were increased. Moreover, organization of the ER network and structure of the actin cytoskeleton were altered in the absence of ANXA2, suggesting that ANXA2 binding to actin is required to maintain ER network morphology. These findings provide evidence that interactions of reovirus nonstructural proteins directly or indirectly with ANXA2 are required for ER remodeling and formation of the membranous fragments that serve as the matrix to build reovirus factories

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ORGANISM(S): Reovirus Sp. Homo Sapiens (human)

SUBMITTER: Sergio Ciordia

LAB HEAD: Cristina Risco

PROVIDER: PXD067290 | Pride | 2025-10-29

REPOSITORIES: Pride

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			Action	DRS
	F113078.mgf	Mgf
	F113078.mzML	Mzml
	F113078.mzid	Mzid
	F113080.mgf	Mgf
	F113080.mzML	Mzml

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Annexin A2 stabilizes the endoplasmic reticulum and actin cytoskeleton and regulates formation of reovirus factories

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