Dataset Information

Medulloblastoma LC-MS/MS using small extracellular vesicles from blood plasma, CSF, and MB cell lines

ABSTRACT: Liquid biopsy is increasingly recognized for tumor diagnosis and monitoring, and in medulloblastoma (MB) and other CNS tumors, it offers the potential to bypass invasive tissue collection. However, its application in clinical practice remains limited, in part because the optimal choice of biofluid is underexplored and may differ between tumor types. Here, we report mass spectrometry–based proteomic analysis of extracellular vesicles (EVs) from three matched cerebrospinal fluid (CSF) and blood plasma (BP) samples of MB patients, alongside healthy BP controls and MB cell lines.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma, Cerebrospinal Fluid, Cell Culture

DISEASE(S): Brain Cancer

SUBMITTER: Frank Stein

LAB HEAD: Basant Kumar Thakur

PROVIDER: PXD067299 | Pride | 2025-09-22

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	F116639.dat	Other
	F116640.dat	Other
	P2258_Zelda_220909_P2258_MR_LR_EVs_TMT12_1out80_R1_results_20220912_1012_peptides.txt	Txt
	P2258_Zelda_220909_P2258_MR_LR_EVs_TMT12_1out80_R1_results_20220912_1012_proteins.txt	Txt
	P2258_Zelda_220909_P2258_MR_LR_EVs_TMT12_1out80_R1_results_20220912_1012_summary.txt	Txt

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Project description:Environmental enrichment (EE) conditions have profound beneficial effects for reinstating cognitive ability in neuropathological disorders like Alzheimerâs disease (AD). While EE benefits involve epigenetic gene control mechanisms that comprise histone acetylation, the histone acetyltransferases (HATs) involved remain largely unknown. Here, we examine a role for Tip60 HAT action in mediating activity- dependent beneficial neuroadaptations to EE using the Drosophila CNS mushroom body (MB) as a well-characterized cognition model. We show that flies raised under EE conditions display enhanced MB axonal outgrowth, synapse protein production, histone acetylation induction and transcriptional activation of cognition linked genes when compared to their genotypically identical siblings raised under isolated conditions. Further, these beneficial changes are impaired in both Tip60 HAT mutant flies and APP neurodegenerative flies. While EE conditions provide only slight beneficial neuroadaptive changes in the APP neurodegenerative fly MB, such positive changes are significantly enhanced by increasing MB Tip60 HAT levels. Our results implicate Tip60 as a critical mediator of EE-induced benefits, and provide insight into synergistic behavioral and epigenetic based approaches for treatment of cognitive disorders. EE has been shown to positively impact gene expression profiles in the mouse brain that are enriched in functions such as neuronal structure, synaptic plasticity and neurotransmission. Thus, we asked whether the EE induced beneficial MB structural and synaptic changes we observe are accompanied by neuroadaptive transcriptional benefits in the Drosophila MB, and if so, is Tip60 HAT action required for this process. To address this question, we accessed EE induced beneficial transcriptional changes using microarray. We crossed our UAS-mCD8-GFP;Tip60E431Q flies or control UAS-mCD8-GFP flies to MB GAL4 OK-107 to simultaneously induce Tip60 HAT loss in the MB while tagging MB cells with GFP. Adult progeny were exposed to EE or ISO conditions. After conditioning, the GFP tagged MB Kenyon neurons were FACs purified from conditioned fly brains from each genotype to enrich for detection of an EE induced MB transcriptional response. RNA was isolated from the purified Kenyon MB neurons and transcriptional changes for each genotype were assessed using microarray analysis

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Medulloblastoma LC-MS/MS using small extracellular vesicles from blood plasma, CSF, and MB cell lines

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