Protein-protein interaction network mapping for yeast PKA catalytic subunits using BioID

ABSTRACT: Paralogs engage in biological processes through both redundant and non-redundant functions. In yeast Saccharomyces cerevisiae, approximately one-fifth of the genome consists of paralogs, with their encoded proteins involved in multiple crucial pathways. However, the contributions of individual paralogs have remained poorly defined. Here, we undertook a systematic examination of eight paralog pairs in the glucose-sensing pathway, including identifying protein-protein interactions (PPIs) through proteomic study. Focusing on the PKA catalytic subunits, comprised of the paralogs Tpk1 and Tpk3 as well as the isomorphic kinase Tpk2, we employed BioID to identify unique and shared binding partners. Our data reveal that, through different PPIs, paralogs in the glucose-sensing pathway contribute in multiple and unique ways to signal transduction, and establish potential mechanisms driving the preservation of these and other duplicated genes.

INSTRUMENT(S):

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Scott Lyons  

LAB HEAD: Henrik Dohlman

PROVIDER: PXD067402 | Pride | 2026-09-28

REPOSITORIES: Pride