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Proteome of wild type and Nsun7 knockout mice spermatozoa

ABSTRACT: Investigation of a new RNA-modification-based mechanism in mammalian spermatogenesis. We created a mice line devoid of testis-specific m5C RNA methyltransferase Nsun7. These mice demonstrated male infertility due to a peculiar defect in sperm tail assembly – mispositioning of the longitudinal columns of the fibrous sheath. The spermatozoa proteomes of wild type and Nsun7 knockout mice were obtained to study the molecular pathways affected by NSUN7.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Testis, Sperm

DISEASE(S): Infertility

SUBMITTER: Ekaterina Guseva

LAB HEAD: Petr Sergiev

PROVIDER: PXD067410 | Pride | 2026-01-12

REPOSITORIES: Pride

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			Action	DRS
	130224LSB_Nsun7-_04_20240214011754.raw	Raw
	130224LSB_Nsun7-_04_20240214022408.raw	Raw
	130224LSB_Nsun7-_04_20240215201556.raw	Raw
	130224LSB_Nsun7-_04_20240216220407.raw	Raw
	130224LSB_Nsun7-_04_20240216231019.raw	Raw

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Proteome of wild type and Nsun7 knockout mice testis

Project description:Investigation of a new RNA-modification-based mechanism in mammalian spermatogenesis. We created a mice line devoid of testis-specific m5C RNA methyltransferase Nsun7. These mice demonstrated male infertility due to a peculiar defect in sperm tail assembly – mispositioning of the longitudinal columns of the fibrous sheath. The whole testis proteomes of wild type and Nsun7 knockout mice were obtained to study the molecular pathways affected by NSUN7.

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Positioning of sperm tail longitudinal columns depend on Nsun7, an RNA binding protein destabilizing elongated spermatid transcripts (CLIP-Seq)

Project description:Spermatozoid’s flagella assemble in transcriptionally silent spermatids and thus depends on post-transcriptional regulation of gene expression. Mutations in Nsun7 gene are known to cause male infertility in human and mice. We identified m5C-specific Nsun7 RNA methyltransferase as a protein present in elongated spermatids and interacting with RNAs specific for this type of spermatozoid’s precursor cells. Inactivation of Nsun7 gene in mice leads to upregulation of its RNA interactors, thus indicating that Nsun7 downregulates a set of RNAs in the elongated spermatids. A physiologic consequence of Nsun7 gene knockout is male infertility, which is mechanistically explained by the observed mispositioning of longitudinal columns relative to the axonemal microtubular doublets leading to motility defect.

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