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Proteomic Dataset of Non-Specific Interactors in Leishmania infantum

ABSTRACT: This dataset provides a proteomic resource of non-specific protein interactions in Leishmania infantum identified by affinity purification coupled to mass spectrometry (AP-MS/MS). Protein extracts from promastigotes expressing Cas9/T7 RNA polymerase were subjected to immunoprecipitation using HA, myc, and His affinity systems, followed by LC–MS/MS analysis. Raw spectral data were processed using MaxQuant against the L. infantum reference proteome from UniProtKB. The dataset includes raw mass spectrometry files, peptide and protein identification tables, and label-free quantification (LFQ) data, all publicly available via the ProteomeXchange Consortium under the identifier PXD067464. After data filtering, a total of 566 unique proteins were identified across all conditions, including 60 proteins consistently detected in all three affinity systems, representing putative non-specific binders. Among these, metabolism-related proteins (30%) and ribosomal components (28%) were the most abundant functional classes. This dataset provides a valuable reference for background protein binding in AP-MS/MS experiments in Leishmania , supporting improved interpretation of interactome studies and benchmarking of affinity purification strategies.

INSTRUMENT(S):

ORGANISM(S): Leishmania Infantum Jpcm5

DISEASE(S): Visceral Leishmaniasis

SUBMITTER: Wesley Regatieri

LAB HEAD: Felipe Roberti

PROVIDER: PXD067464 | Pride | 2026-05-20

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	All_data_after_MaxQuanttable.xlsx	Xlsx
	Output_maxquant.zip	Other
	SDRF_Metadates.tsv	Tabular
	anti-HA_raw_data_triplicate.zip.zip	Other
	anti-myc_raw_data_triplicate.zip.zip	Other

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