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Mass Spectrometry on NUP153(NG)AID cells infected with WNV

ABSTRACT: The nuclear pore complex is built of multiple copies of different nucleoporins (NUP), that together enable the transport across the nuclear envelope. One of the NUPs is NUP153, which primarily is involved in the shuttling of RNA, yet also has functions in the assembly and structure of the pore. We have implemented the auxin inducible degron (AID) system to rapidly deplete NUP153 in cells and evaluate the impact this has on orthoflavivirus infection. To determine which viral and cellular proteins are impacted by NUP153 depletion we have performed mass spectrometry experiments in AID and control cells infected with West Nile virus, to help elucidate the molecular mechanism of NUP153 in orthoflavivirus infection.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human) West Nile Virus

TISSUE(S): Cell Culture, Embryonic Stem Cell

SUBMITTER: Klaus Faserl

LAB HEAD: Klaus Faserl

PROVIDER: PXD067546 | Pride | 2026-03-05

REPOSITORIES: Pride

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			Action	DRS
	17_NoAUX_WNV_1_WNV_500ng.msf	Msf
	17_NoAUX_WNV_1_WNV_500ng.raw	Raw
	18_NoAUX_WNV_2_WNV_500ng.msf	Msf
	18_NoAUX_WNV_2_WNV_500ng.raw	Raw
	19_NoAUX_WNV_3_WNV_500ng.msf	Msf

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