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The rapid manufacture of biologics using mRNA

ABSTRACT: We evaluate bevacizumab, a monoclonal antibody, expression in time course from mRNA transient transfection in CHO cells using MS. We transiently transfected bevacizumab heavy and light chain mRNAs into CHO-S WT using lipoplex. Later, we analysed the bevacizumab protein intracellularly.

INSTRUMENT(S):

ORGANISM(S): Cricetulus Griseus (chinese Hamster) (cricetulus Barabensis Griseus)

TISSUE(S): Cell Culture

SUBMITTER: Thornwit Chavalparit

LAB HEAD: Esteban Marcellin

PROVIDER: PXD067559 | Pride | 2025-12-22

REPOSITORIES: Pride

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Rapid expression of therapeutic antibodies in mammalian cells via mRNA transfection.

Chavalparit Thornwit T Barry Craig C Gunter Helen H Gillard Marianne M Mercer Timothy T Marcellin Esteban E

mAbs 20251212 1

Messenger RNA (mRNA) has emerged as a powerful tool for protein expression in clinical settings, yet its potential as a platform for biologics manufacturing remains underexplored. Here, we evaluate transient mRNA transfection in Chinese hamster ovary (CHO) cells as a rapid and versatile system for protein production. Using reporter mRNAs, we optimize transfection efficiency and benchmark performance against industry-standard plasmid transfection and stable cell line methods. We demonstrate that ...[more]

PMID: 41384441

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