Proteomic and functional profiling of suspended jejunal enteroids reveals physiological and pharmacokinetic fidelity to native intestine

ABSTRACT: Intestinal enteroids are “mini-guts” that recapitulate essential features of the intestinal epithelium in vitro. We developed and characterized native basal-out (BO) and morphogenically everted apical-out (AO) jejunal enteroids cultured in suspension. Using quantitative proteomics, we show that these enteroids progressively adopt protein profiles closely resembling those of freshly isolated villus enterocytes and human jejunal tissue.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Stem Cell, Enterocyte, Small Intestine Mucosa

SUBMITTER: Merve Ceylan  

LAB HEAD: Per Artursson

PROVIDER: PXD067571 | Pride | 2026-08-21

REPOSITORIES: Pride