Proteomics

Dataset Information

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Identification of 60S pre-rRNA binding sites of human GNL3L GTPase

ABSTRACT: We reveal that the GNL3 paralogue GTPase, GNL3L, associates with pre-ribosomes. We map RNA contact sites on GNL3L as well as GNL3L binding sites on pre-rRNAs. Lack of GNL3L impairs synthesis of the 60S rRNAs and expression of GTPase inactive GNL3L causes defects in early steps of pre-rRNA processing. Impaired GTP hydrolysis by GNL3L leads to its accumulation on pre-60S particles, together with other assembly factors with proximal binding sites.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Flp-in-t-rex Cell

SUBMITTER: Sergei Moshkovskii  

LAB HEAD: Henning Urlaub

PROVIDER: PXD067606 | Pride | 2026-03-05

REPOSITORIES: Pride

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MR_ChThome_Bohnsack_210923_131023_Expl1_RNA-XL_UV1.raw Raw
MR_ChThome_Bohnsack_210923_131023_Expl1_RNA-XL_UV1_perc_0.0100_XLs.idXML Other
MR_ChThome_Bohnsack_210923_131023_Expl1_RNA-XL_UV2.raw Raw
MR_ChThome_Bohnsack_210923_131023_Expl1_RNA-XL_UV2_perc_0.0100_XLs.idXML Other
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Publications

Production of the eukaryotic ribosomal subunits (40S and 60S) is a highly dynamic process in which numerous assembly factors (AFs) coordinate structural rearrangements of pre-ribosomal complexes to achieve their mature, functional architectures. Across the domains of life, GTPases leverage their functions as molecular switches to induce conformational changes that drive key steps in subunit maturation. Three GTPases, GTPBP4, GNL2, and GNL3, have been detected in nucleolar/nucleoplasmic human pre  ...[more]

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