Dataset Information

The mass spectrometry raw data of proteins that interact with GRA35 in neuronal cells

ABSTRACT: To investigate the impact of Toxoplasma gondii GRA35 on neuronal cells and the underlying mechanisms, we conducted GST-pull down experiments to isolate proteins that bind to GRA35-GST and GST in neuronal cells, respectively. The dataset labeled "20240706_WJ_ GRA35_N_B01.raw" corresponds to the GRA35-GST group, whereas "20240706_WJ_C1_B01.raw" represents the data for the GST group. Subsequent mass spectrometry analysis revealed that the neuron-specifically expressed RTN1c protein interacts with GRA35.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Jie Wang

LAB HEAD: Jie Wang

PROVIDER: PXD067653 | Pride | 2025-08-26

REPOSITORIES: Pride

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Source:

			Action	DRS
	20240706_WJ_C1_B01-proteinGroups.txt	Txt
	20240706_WJ_C1_B01.mzML	Mzml
	20240706_WJ_C1_B01.raw	Raw
	20240706_WJ_GRA35_N_B01-proteinGroups.txt	Txt
	20240706_WJ_GRA35_N_B01.mzML	Mzml

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Project description:Signal transduction by the NF-kappaB pathway is a key regulator of a host of cellular responses to extracellular and intracellular messages. The NEMO adaptor protein lies at the top of this pathway and serves as a molecular conduit, connecting signals transmitted from upstream sensors to the downstream NF-kappaB transcription factor and subsequent gene activation. The position of NEMO within this pathway makes it an attractive target from which to search for new proteins that link NF-kappaB signaling to additional pathways and upstream effectors. In this work, we have used protein microarrays to identify novel NEMO interactors. A total of 112 protein interactors were identified, with the most statistically significant hit being the canonical NEMO interactor IKKbeta, with IKKalpha also being identified. Of the novel interactors, more than 30% were kinases, while at least 25% were involved in signal transduction. Binding of NEMO to several interactors, including CALB1, CDK2, SAG, SENP2 and SYT1, was confirmed using GST pulldown assays and coimmunoprecipitation, validating the initial screening approach. Overexpression of CALB1, CDK2 and SAG was found to stimulate transcriptional activation by NF-kappaB, while SYT1 overexpression repressed TNFalpha-dependent NF-kappaB transcriptional activation in human embryonic kidney cells. Corresponding with this finding, RNA silencing of CDK2, SAG and SENP2 reduced NF-kappaB transcriptional activation, supporting a positive role for these proteins in the NF-kappaB pathway. The identification of a host of new NEMO interactors opens up new research opportunities to improve understanding of this essential cell signaling pathway. For microarray screening, Invitrogen Protoarray v4.0 protein microarrays were used. Human NEMO expressed as a C-terminal GST fusion was purified from E. coli lysates and labelled with biotin. NEMO or biotinylated GST were applied to the microarrays and binding partners detected using streptavidin-Alexa Fluor 647. Significant interactors on both arrays were detected using Invitrogen Protoarray Prospector software and a Z-score cutoff of 3.0. Following subtraction of interactors present on the GST control array, a final set of significant NEMO interactors was derived. Full experimental details are supplied in Fenner, B. J., Scannell, M. & Prehn, J. H. M. (2010). Expanding the substantial interactome of NEMO using protein microarrays. PLoS ONE (in press).

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The mass spectrometry raw data of proteins that interact with GRA35 in neuronal cells

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