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Targeted quantitative analysis of specific proteins in cytosolic, mitochondrial, and nuclear fractions using PRM

ABSTRACT: We present a targeted proteomic approach using parallel reaction monitoring (PRM) to assess the enrichment of cytosolic, mitochondrial, and nuclear fractions obtained from liver samples using commercial isolation kits. For mitochondrial fraction, CPS1 and ATP5F1 were chosen. For the cytosolic fraction, HSP90AB1 and AHCY were selected. For the nuclear fraction, LMNA and NPM1 were used as markers.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Primary Cell Line Cell, Liver

SUBMITTER: Alejandra Delgado Sequera

LAB HEAD: Fernando Corrales

PROVIDER: PXD067737 | Pride | 2026-04-13

REPOSITORIES: Pride

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			Action	DRS
	Cytosol_cells_1.raw	Raw
	Cytosol_cells_2.raw	Raw
	Cytosol_cells_3.raw	Raw
	Cytosol_liver_1.raw	Raw
	Cytosol_liver_2.raw	Raw

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Publications

Targeted Quantitative Analysis of Specific Proteins in Cytosolic, Mitochondrial, and Nuclear Fractions Using PRM.

Delgado-Sequera Alejandra A Paradela Alberto A Corrales Fernando J FJ

Journal of proteome research 20260225 4

Mitochondria play a central role in liver physiology by regulating key metabolic processes. Consequently, mitochondrial dysfunction is a hallmark of multiple liver diseases, including steatosis, steatohepatitis, and liver failure following hepatectomy. Subcellular fractionation is widely used to isolate mitochondria from liver cells or tissue; however, the enrichment and purity of isolated fractions are critical to ensure reliable downstream functional and proteomic analyses. Conventional valida ...[more]

PMID: 41740191

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