Dataset Information

Unveiling Dopamine and Met-Enkephalin Dynamics: Simultaneous Co-Detection in Rat Striatum

ABSTRACT: Project description Fast -scan cyclic voltammetry was coupled with carbon microelectrodes for co-detection of dopamine and met-enkephalin at single recording sites in rat striatal slices. The measurements used a specifically designed voltammetric waveform to minimize sensitivity to dopamine, maximize sensitivity to enkephalin, and to minimize biofouling. Both neurotransmitter (dopamine) and neuropeptide (enkephalin) release scaled with stimulation duration. Enkephalin dynamics displayed a unique biphasic profile with a significant latency to peak that occurred ~30 seconds after stimulation. The data suggests a sphere of influence that is ~3x larger than that of dopamine and that is consistent with multiple forms of the peptide released at once. Signals were validated by mass spectrometry of collected striatal relesates. The findings provide direct evidence to support widely held assumptions regarding neuropeptide release, and they demonstrate how these signaling molecules can affect distinct cellular populations in striatum, even when recorded at the same site.

INSTRUMENT(S):

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Brain

SUBMITTER: Elena Romanova

LAB HEAD: Jonathan V. Sweedler

PROVIDER: PXD067765 | Pride | 2025-12-22

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	JB079_StriatumA1_20Hz20Pl_Slot1-46_1_302.d.mzxml	Mzxml
	JB079_StriatumA1_20Hz20Pl_Slot1-46_1_302.d.refined.mgf	Mgf
	JB079_StriatumA1_20Hz20Pl_Slot1-46_1_302.d.zip	Other
	JB079_StriatumA2_20Hz60Pl_Slot1-47_1_303.d.mzxml	Mzxml
	JB079_StriatumA2_20Hz60Pl_Slot1-47_1_303.d.refined.mgf	Mgf

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Publications

Unveiling Dopamine and Met-Enkephalin Dynamics: Simultaneous Co-Detection in Rat Striatum.

Berger Jenna M JM Todorov Jovica J Turner Kalynn M KM McCarty Gregory S GS Romanova Elena V EV Sweedler Jonathan V JV Sombers Leslie A LA

Analytical chemistry 20251108 45

Endogenous opioid peptides have been linked to numerous physiological functions, including pain perception and the motivational drive associated with substance use disorders, but many fundamental aspects of transmission remain ambiguous. The kinetics of endogenous opioid peptides are thought to be slower and to last longer than those of more classical small-molecule neurotransmitters, like dopamine; however, a direct comparison of the release and diffusive spread of these molecules in the brain ...[more]

PMID: 41204894

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