Dataset Information

Proteomic Insights into the Antibacterial Mechanism of Brevibacillus brevis HK544 and Edeine B1 against Erwinia amylovora

ABSTRACT: - Identification of proteins whose expression was affected by B. brevis culture filtrate (CF) and purified EB1 in Erwinia amylovora str. TS3128 - Shotgun proteomic analysis was used - Three conditions were used with three biological replicates (total 9 samples). CK: no treatment (control). CF: treatment of B. brevis culture filtrate. EB : treatment of EB

INSTRUMENT(S):

ORGANISM(S): Erwinia Amylovora

TISSUE(S): Cell Suspension Culture, Cell Culture

SUBMITTER: Sang-Wook Han

LAB HEAD: Sang-Wook Han

PROVIDER: PXD067784 | Pride | 2026-04-13

REPOSITORIES: Pride

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Proteomic Insights into the Antibacterial Mechanism of Brevibacillus brevis HK544 Producing Edeine B1 against Erwinia amylovora.

Kim Yeong Seok YS Rhyu Haerim H Kim Bomin B Van Nguyen Minh M Han Jae Woo JW Choi Gyung Ja GJ Son Hokyoung H Han Sang-Wook SW Kim Hun H

The plant pathology journal 20260225 2

Recently, we reported that Brevibacillus brevis HK544, which produces edeine B1 (EB1), exhibits promising antimicrobial activity against plant pathogens. To elucidate its antibacterial mechanisms against Erwinia amylovora, the causal agent of fire blight, we conducted label-free shotgun proteomic analysis of E. amylovora TS3128 treated with B. brevis culture filtrate (CF) or purified EB1. Comparative analysis showed significant (over twofold) changes in 45 of 624 and 81 of 846 quantified protein ...[more]

PMID: 41742721

Similar Datasets

Project description:Bacillus thuringiensis, a well-known and effective bio-insecticide, has attracted considerable attention as a potential biological control agent for the suppression of plant diseases. Treatment of tomato roots with a filter-sterilized cell-free filtrate (CF) of B. thuringiensis systemically suppresses bacterial wilt caused by Ralstonia solanacearum through systemic activation of the plant defense system. Comparative analysis of the expression of the Pathogenesis-Related 1(P6) [PR-1(P6)] gene, a marker for induced resistance to pathogens, in various tissues of tomato plants treated with CF on their roots suggested that the B. thuringiensis-induced defense system was activated in the leaf, stem, and main root tissues, but not in the lateral root tissue. At the same time, the growth of R. solanacearum was significantly suppressed in the CF-treated main root tissue but not in the CF-treated lateral root tissue. This distinct activation of the defense reaction and suppression of R. solanacearum were reflected by the differences in the transcriptional profiles of the main and lateral tissues in response to the CF. In the CF-treated main root tissue, but not CF-treated lateral root tissue, the expression of several salicylic acid (SA)-responsive defense-related genes was specifically induced, whereas jasmonic acid (JA)-related gene expression was either down-regulated or not induced in response to the CF. On the other hand, genes encoding ethylene (ET)-related proteins were induced equally in both the main and lateral root tissues. Taken together, the co-activation of SA-dependent signaling pathway with ET-dependent signaling pathway and suppression of JA-dependent signaling pathway may play key roles in B. thuringiensis-induced resistance to R. solanacearum in tomato plants. Gene expression was measured in main and lateral root tissues of tomato treated with Bacillus thuringiensis or distilled water-treated control at 48 hours after treatment. Two independent experiments were performed at each tissue (main root or lateral root tissue) for each treatment (Bacillus thuringiensis or distilled water control).

			Action	DRS
	CF_1.msf	Msf
	CF_1.raw	Raw
	CF_2.msf	Msf
	CF_2.raw	Raw
	CF_4.msf	Msf