Dataset Information

Identify proteins that interact with hsa_circ_0001910 using mass spectrometry.

ABSTRACT: Circular RNAs (circRNAs) have emerged as critical regulators in various biological processes through their interactions with RNA-binding proteins. To investigate the protein interactions of hsa_circ_0001910, a circRNA of potential functional significance, we designed a specific RNA pulldown probe targeting this molecule. Using this probe, we performed RNA pull-down assays to enrich proteins that directly or indirectly bind to hsa_circ_0001910. The pulled-down protein complexes were subsequently separated and detected by Western blotting to confirm the presence of specific protein candidates. For comprehensive and unbiased protein identification, the enriched protein samples were further subjected to mass spectrometry (MS)-based proteomic analysis. This approach allows for the systematic identification and quantification of proteins that interact with hsa_circ_0001910, providing insights into its molecular functions and potential roles in cellular pathways.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Fei Liu

LAB HEAD: Fei Liu

PROVIDER: PXD067788 | Pride | 2025-11-03

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	T480_FAIMS_JD_SHY005_hsa_circ_0001910.raw	Raw
	checksum.txt	Txt
	txt.zip	Other

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Publications

circWWC3 enhances the progression of triple-negative breast cancer by interacting with vimentin to regulate the secretion of CSF2.

Wu Ming M Du Yifan Y Dong Yuyang Y Zheng Yang Y Gu Lina L Tang Xiaojun X Yan Li L Ji Hong H Sang Yang Y Liu Fei F

Frontiers in immunology 20251008

<h4>Introduction</h4>Circular RNAs (circRNAs) have been reported to be important in the development and progression of breast cancer. Nevertheless, the biological functions and mechanisms underlying the action of circRNAs in triple-negative breast cancer (TNBC) remain poorly understood. The present study aimed to explore the role of hsa_circ_0001910 (also termed circWWC3) interacting with vimentin in regulating the secretion of Colony Stimulating Factor 2 (CSF2) and its effects on the malignant ...[more]

PMID: 41132646

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