Dataset Information

Profiling Neuropeptides in Adrenomedullary Chromaffin Cells: Insights into Regulated Release via Electrical and Chemical Stimulation

ABSTRACT: Despite longstanding recognition that the adrenal medulla is a major source of catecholamines and opioid peptides, the dynamic co-release of these signaling molecules—and the full complexity of their molecular forms—remains poorly understood. This study addresses this gap by integrating real-time electrochemical detection with tandem mass spectrometry to map and quantify the neurochemical complexity of opioid peptide signaling from adrenal chromaffin cells. Using FSCV, we provide evidence for stimulus-evoked co-release of met-enkephalin and catecholamines from single chromaffin cells (and adrenal slices?). With MS, we show that proenkephalin-derived peptides dominate the adrenal peptidome and that extended enkephalin peptides—such as BAM-18 and BAM-22—are not only present in cell content but are actively released.

INSTRUMENT(S):

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Adrenal Medulla

SUBMITTER: Elena Romanova

LAB HEAD: Jonathan V. Sweedler

PROVIDER: PXD067807 | Pride | 2026-06-29

REPOSITORIES: Pride

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Publications

Profiling Endogenous Opioid Peptide Release from Adrenal Chromaffin Cells.

Berger Jenna M JM Denison J Dylan JD De Alwis A Chathuri AC Sumner Hannah P HP McCarty Gregory S GS Romanova Elena V EV Sweedler Jonathan V JV Sombers Leslie A LA

ACS chemical neuroscience 20260506 10

Despite longstanding recognition that the adrenal medulla is a major source of both catecholamines and opioid peptides, direct measurements of opioid peptide release kinetics─and characterization of the full complexity of the peptidergic forms released─remain under studied. Here, this gap is addressed by introducing a workflow that integrates real-time electrochemical detection of catecholamine (CA) and enkephalin (ENK) release kinetics with tandem mass spectrometry to profile opioid peptide sig ...[more]

PMID: 42089390

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Project description:Stress leads to important adaptive physiological responses including release of catecholamines from the adrenal medulla. Repeated or prolonged stress increases the propensity for many disorders including cardiovascular disease and neuropsychiatric disorders. The susceptibility to stress is determined by a number of factors including prior experience with stress. It has been found that while there is adaptation to prolonged cold stress; it leads to sensitization when cold stressed animals are exposed to a different (novel) stressor. Our study aims to determine the mechanism of this adaptation and sensitization.,We will determine the gene expression patterns in rat adrenal medulla in response to several times of cold stress. We will also determine the changes in gene expression with single immobilization stress in adrenal medulla of rat with and without preexposure to cold stress to investigate the mechanism of sensitization in cold adapted animals. ,We hypothesize that during the course of cold stress there are alterations in gene expression of several neurotransmitter related genes and transcription factors. In cold adapted animals, there is increased susceptibility to stress due to sustained changes in expression of several transcription factors, such as FRA-2 which lead to an exaggerated response to the novel stressor. ,Rats were exposed to cold stress (4oC) for 1, 7 or 28 days. A parallel group was kept at room temperature (unstressed). Unstressed or rats pre-exposed to 28 days cold were immobilized for 2 hrs and killed by decapitation. The adrenal medullae, separately from right and left side, have been dissected and individually frozen at -70oC. Our recent immunoblots with the tissue from one side revealed that there is increase in tyrosine hydroxylase and c-fos with the short term cold stress and in dopamine beta hydroxylase and Fra-2 even with the prolonged cold stress. The response of several transcription factors to immobilization stress was enhanced in the cold adapted animals. RNA will be prepared with RNAqueous-Micro Isolation kit from Ambion (alternatively we can ship the tissues). We will pool samples from 4 rats per treatment to control for variability.

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