Dataset Information

Adaptive Responses of Genome-Reduced Bacillus subtilis During Enzyme Secretion in a Breathing Vessel Culture

ABSTRACT: The utilisation of microbes for the production of enzymes and pharmaceutical proteins is an important step towards a sustainable future. However, industrial fermentation processes require energy for agitation, aeration and cooling. This energy requirement increases with the increasing microbial cell mass. To address this technological challenge, we previously developed a so-called ‘breathing’ fermentation vessel that allows effective gas exchange. The present study aimed to explore the potential application of the breathing vessel for enzyme production by the Gram-positive bacterial cell factory Bacillus subtilis. In particular, we compared production of the secreted α-amylase AmyQ by the genome-reduced B. subtilis strain IIG-Bs-27-31 during parallel culturing in breathing vessels and shake flasks. Enzyme yields and the cellular and extracellular proteome compositions were compared.

INSTRUMENT(S):

ORGANISM(S): Bacillus Subtilis Subsp. Subtilis Str. 168

SUBMITTER: Sandra Maass

LAB HEAD: Dörte Becher

PROVIDER: PXD067838 | Pride | 2026-02-23

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	240214_VE1_PM4_PG_SK_2024_003_Japan_T8_Fermenter_SN_BR1.raw	Raw
	240214_VE1_PM4_PG_SK_2024_003_Japan_T8_Fermenter_SN_BR2.raw	Raw
	240214_VE1_PM4_PG_SK_2024_003_Japan_T8_Fermenter_SN_BR3.raw	Raw
	240214_VE1_PM4_PG_SK_2024_003_Japan_T8_Fermenter_SN_BR4.raw	Raw
	240214_VE1_PM4_PG_SK_2024_003_Japan_T8_Shake_SN_BR1.raw	Raw

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Adaptive responses of genome-reduced Bacillus subtilis during enzyme secretion in a breathing vessel culture.

Öktem Ayşegül A Yokoyama Kyosuke K Wu Yuzheng Y Grilli Francesca F Maaß Sandra S Ishikawa Shu S Yotsuya Masato M Sato Ryosuke R van Dijl Jan Maarten JM Yoshida Ken-Ichi KI

Microbial cell factories 20260129

<h4>Background</h4>The utilisation of microbes for the production of enzymes and pharmaceutical proteins is an important step towards a sustainable future. However, the energy requirement for agitation, aeration and cooling during industrial fermentation processes is substantial. To address this challenge, we have previously developed a 'breathing' polytetrafluoroethylene fermenter vessel that allows effective gas exchange with ambient air and, thus, does not require sparger aeration. The presen ...[more]

PMID: 41612395

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