Dataset Information

Standardized Sample Preparation of Paediatric Bronchoalveolar Lavage Fluid for Mass Spectrometry-based Proteomic Analysis

ABSTRACT: This study establishes a standardized and reproducible workflow for LC-MS/MS-based proteomic analysis of paediatric bronchoalveolar lavage fluid (BALF). Using minimal sample volumes, we systematically compared preparation strategies and identified a simplified protocol that maximizes protein recovery and coverage, supporting future large-scale biomarker and mechanistic studies in paediatric respiratory diseases.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Bronchoalveolar Lavage

SUBMITTER: Gereon Poschmann

LAB HEAD: Gereon Poschmann

PROVIDER: PXD067840 | Pride | 2025-11-21

REPOSITORIES: Pride

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Standardized sample preparation of paediatric bronchoalveolar lavage fluid for mass spectrometry based proteomic analysis.

Freitag Nadine N Schramm Dirk D Stefanski Anja A Schroeter Christina B CB Stühler Kai K Poschmann Gereon G Driessen Marc D MD

Molecular and cellular pediatrics 20251120 1

BACKGROUND: Bronchoalveolar lavage fluid (BALF) is a valuable diagnostic and research tool in paediatric respiratory medicine. Mass spectrometry-based proteomic analysis of BALF can contribute to uncover disease mechanisms and biomarkers, but standardized protocols for paediatric BALF are lacking. This study aimed to establish a robust, reproducible workflow for liquid chromatography-tandem mass spectrometry (LC-MS/MS) of paediatric BALF and to evaluate its applicability in samples from patients ...[more]

PMID: 41264199

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