Standardized Sample Preparation of Paediatric Bronchoalveolar Lavage Fluid for Mass Spectrometry-based Proteomic Analysis

ABSTRACT: This study establishes a standardized and reproducible workflow for LC-MS/MS-based proteomic analysis of paediatric bronchoalveolar lavage fluid (BALF). Using minimal sample volumes, we systematically compared preparation strategies and identified a simplified protocol that maximizes protein recovery and coverage, supporting future large-scale biomarker and mechanistic studies in paediatric respiratory diseases.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Bronchoalveolar Lavage

SUBMITTER: Gereon Poschmann  

LAB HEAD: Gereon Poschmann

PROVIDER: PXD067840 | Pride | 2025-11-21

REPOSITORIES: Pride