Dataset Information

Secretome of hTERT-Immortalized Human Mesenchymal Stem Cells

ABSTRACT: The secretome of mesenchymal stromal cells (MSCs) can efficiently stimulate regener-ation and therefore is a tempting remedy for “cell-free cellular therapy”. However, the usage of primary MSC cultures as secretome-producers for translation studies has ob-vious obstacles, including rapid aging of MSC cultures, the need for a large number of verified donors and donor-to-donor variability of secretome content. MSCs immortal-ization allows to overcome those limitations and to obtain secretome-producing cul-tures with prolonged lifetime. However, the efficacy and safety of such secretomes are critical issues, which limit their usage as therapeutic agents. In this study we have tested in large detail how the immortalization of MSC cultures affects the content, bi-ological activity and safety of their secretome. MSCs immortalization via overexpres-sion of human TERT gene does not significantly alter the qualitative and quantitative composition of their secretome or its activity according to the results of proteomic analysis, ELISA, qPCR and functional tests in vitro. Moreover, we have demonstrated that the secretome of immortalized MSCs does not contain detectable amounts of te-lomerase and does not possess any transforming activity. Altogether, our data suggest that immortalized MSC cultures may become a reliable source for obtaining standard-ized active secretome in large-scale quantities for clinical use.The secretome of mesenchymal stromal cells (MSCs) can efficiently stimulate regener-ation and therefore is a tempting remedy for “cell-free cellular therapy”. However, the usage of primary MSC cultures as secretome-producers for translation studies has ob-vious obstacles, including rapid aging of MSC cultures, the need for a large number of verified donors and donor-to-donor variability of secretome content. MSCs immortal-ization allows to overcome those limitations and to obtain secretome-producing cul-tures with prolonged lifetime. However, the efficacy and safety of such secretomes are critical issues, which limit their usage as therapeutic agents. In this study we have tested in large detail how the immortalization of MSC cultures affects the content, bi-ological activity and safety of their secretome. MSCs immortalization via overexpres-sion of human TERT gene does not significantly alter the qualitative and quantitative composition of their secretome or its activity according to the results of proteomic analysis, ELISA, qPCR and functional tests in vitro. Moreover, we have demonstrated that the secretome of immortalized MSCs does not contain detectable amounts of te-lomerase and does not possess any transforming activity. Altogether, our data suggest that immortalized MSC cultures may become a reliable source for obtaining standard-ized active secretome in large-scale quantities for clinical use.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Stem Cell

SUBMITTER: Elizaveta Chechekhina

LAB HEAD: Maxim N. Karagyaur

PROVIDER: PXD067843 | Pride | 2025-10-27

REPOSITORIES: Pride

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Safety and Regenerative Properties of Immortalized Human Mesenchymal Stromal Cell Secretome.

Karagyaur Maxim M Primak Alexandra A Basalova Nataliya N Monakova Anna A Tolstoluzhinskaya Anastasia A Kulebyakina Maria M Chechekhina Elizaveta E Skryabina Mariya M Grigorieva Olga O Chechekhin Vadim V Yakovleva Tatiana T Turilova Victoria V Shagimardanova Elena E Gazizova Guzel G Vigovskiy Maksim M Kulebyakin Konstantin K Sysoeva Veronika V Dyachkova Uliana U Dzhauari Stalik S Bozov Kirill K Popov Vladimir V Akopyan Zhanna Z Efimenko Anastasia A Kalinina Natalia N Tkachuk Vsevolod V

International journal of molecular sciences 20250924 19

The secretome of mesenchymal stromal cells (MSCs) can efficiently stimulate regeneration and therefore is a tempting remedy for "cell-free cellular therapy". However, the usage of primary MSC cultures as secretome producers for translation studies has obvious obstacles, including the rapid aging of MSC cultures, the need for a large number of verified donors, and donor-to-donor variability of secretome content. MSCs immortalization makes it possible to overcome those limitations and to obtain se ...[more]

PMID: 41096593

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