Dataset Information

Comparative Proteomic Analysis of Extracellular Vesicles from Donkey Colostrum and Mature Milk

ABSTRACT: Donkey milk (DM) has been considered a valuable alternative to human and bovine coun-terparts as well as to infant formulas. Milk extracellular vesicles (EVs) have been proposed to influence key biological processes. The purpose of this study is to provide a compre-hensive characterization of the protein composition of extracellular vesicles (EVs) by ex-tending quantitative proteomic comparisons to EVs derived from donkey colostrum (DC) and mature donkey milk (MDM). The EVs were isolated from DC and MDM samples, characterized, and subjected to proteomic analysis using the tandem mass tag-based quantitative approach

INSTRUMENT(S):

ORGANISM(S): Equus Asinus (donkey)

TISSUE(S): Colostrum, Milk

SUBMITTER: anna maria salzano

LAB HEAD: Dott.Andrea Scaloni

PROVIDER: PXD067848 | Pride | 2025-10-06

REPOSITORIES: Pride

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Comparative Proteomic Analysis of Extracellular Vesicles from Donkey Colostrum and Mature Milk.

Caira Simonetta S Buratta Sandra S Vincenzetti Silvia S Latella Raffaella R Seccaroni Matteo M De Pascale Sabrina S Federici Cristina C Lugini Luana L Giovagnoli Stefano S Salzano Anna Maria AM Emiliani Carla C Scaloni Andrea A Chiaradia Elisabetta E

Metabolites 20250918 9

<b>Background/Objectives:</b> Donkey milk (DM) has been considered a valuable alternative to human and bovine counterparts as well as to infant formulas. Milk extracellular vesicles (EVs) have been proposed to influence key biological processes. The purpose of this study is to provide a comprehensive characterization of the protein composition of extracellular vesicles (EVs) by extending quantitative proteomic comparisons to EVs derived from donkey colostrum (DC) and mature donkey milk (MDM). <b ...[more]

PMID: 41003002

Similar Datasets

Project description:Background: Acute coronary syndromes (ACS) are associated with aberrant gene expression and epigenetic mechanisms. In particular, de novo DNA methylation is typically linked to gene silencing, but its role in heart disease remains not fully understood. Extracellular vesicles (EVs) are active components in cellular communication for their ability to carry a plethora of signalling biomolecules, thus representing a promising new diagnostic/therapeutic approach in cardiovascular diseases (CVDs). Indeed, there is the need of novel biomarkers for ACS prediction and timely detection. Purpose: We hypothesized that specific epigenetic signals can be carried by EVs. In this regard, we isolated and characterized circulating EVs from ACS patients and evaluated their potential role to influence DNA methylation in target cells. Methods: Circulating EVs were recovered, by ultracentrifugation, from plasma samples of 19 ACS patients and 50 healthy subjects (HS). Nanoparticle tracking analysis (NTA) and western blot (WB) were used to confirm the EVs integrity and purity. Peripheral blood mononuclear cells (PBMCs) of volunteer donors were treated with both ACS and HS derived EVs and genomic DNA was extracted to perform epigenome wide analysis through Reduced Representation Bisulfite Sequencing. ShinyGO, PANTHER, and STRING tools were interrogated to perform GO and PPI network analyses. Flow Cytometry, qRT-PCR, and WB analysis were also performed to evaluate and validate both intra-vesicular and intra-cellular signals. Results: Plasma ACS-derived EVs showed a significant up-regulation of DNA methyltransferases (DNMTs) gene expression levels as compared to HS (P<0.001). Specifically, de novo methylation transcripts, as DNMT3A and DNMT3B were significantly increased in plasma ACS-EVs. DNA methylation analysis of PBMCs from volunteer donors treated with HS- and ACS-derived EVs showed that RNF166 and CCND3 genes resulted the most hyper- and hypo-methylated, respectively, after by ACS-EV treatment. In addition, PPI network analysis specifically evidenced the subnetwork with SRC, as a hub gene, connecting it to NOTCH1, FOXO3, CDC42, IKBKG, RXRA, DGKG, known as important genes already involved in the onset of CVDs. Surprising, other novel genes, BAIAP2, SYP, CHL1, and SHB, which were hypomethylated, were found significantly overexpressed in PBMCs (P<0.005), underlying the fundamental modulating properties of EV cargo in atherosclerosis. Conclusions: These findings support the significant role of ACS plasma-derived EVs, inducing de novo DNA methylation signals, and modulating specific signaling pathways in target cells.

			Action	DRS
	TMT-DonkeyExosome_.msf	Msf
	TMT-DonkeyExosome_1.raw	Raw
	TMT-DonkeyExosome_2.raw	Raw
	TMT-DonkeyExosome_3.raw	Raw
	TMT-DonkeyExosome_4.raw	Raw